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Jossé, Lyne and Zhang, Lin and Smales, Christopher Mark (2018) Application of microRNA Targeted 3?UTRs to Repress DHFR Selection Marker Expression for Development of Recombinant Antibody Expressing CHO Cell Pools. Biotechnology Journal, 13 (10). p. 1800129. ISSN 1860-6768. (doi:https://doi.org/10.1002/biot.201800129) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

Vito, Davide and Smales, Christopher Mark (2018) The Long Non-Coding RNA Transcriptome Landscape in CHO Cells Under Batch and Fed-Batch Conditions. Biotechnology Journal, 13 (10). p. 1800122. ISSN 1860-6768. (doi:https://doi.org/10.1002/biot.201800122) (Full text available)

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Gourbatsi, Evdoxia and Povey, Jane F. and Smales, Christopher Mark (2018) The effect of formulation variables on protein stability and integrity of a model IgG4 monoclonal antibody and translation to formulation of a model ScFv. Biotechnology Letters, 40 (1). pp. 33-46. ISSN 0141-5492. E-ISSN 1573-6776. (doi:https://doi.org/10.1007/s10529-017-2443-x) (Full text available)

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Godfrey, Charlotte and Mead, Emma J and Daramola, Olalekan and Dunn, Sarah and Hatton, Diane and Field, Ray and Pettman, Gary and Smales, Christopher Mark (2017) Polysome Profiling of mAb Producing CHO Cell Lines Links Translational Control of Cell Proliferation and Recombinant mRNA Loading onto Ribosomes with Global and Recombinant Protein Synthesis. Biotechnology Journal, 12 (8). p. 1700177. ISSN 1860-6768. E-ISSN 1860-7314. (doi:https://doi.org/10.1002/biot.201700177) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

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Almeida, Aline G. and Pinto, Rodrigo C. V. and Smales, Christopher Mark and Castilho, Leda R. (2017) Investigations into, and development of, a lyophilized and formulated recombinant human factor IX produced from CHO cells. Biotechnology Letters, 39 (8). pp. 1109-1120. ISSN 0141-5492. E-ISSN 1573-6776. (doi:https://doi.org/10.1007/s10529-017-2353-y) (Full text available)

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Migani, Damiano and Smales, Christopher Mark and Bracewell, Daniel G. (2017) Effects of lysosomal biotherapeutic recombinant protein expression on cell stress and protease and general host cell protein (HCP) release in Chinese hamster ovary cells. Biotechnology Progress, 33 (3). pp. 666-676. ISSN 8756-7938. E-ISSN 1520-6033. (doi:https://doi.org/10.1002/btpr.2455) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

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Bastide, Amandine and Peretti, Diego and Knight, John R.P. and Grosso, Stefano and Spriggs, Ruth V. and Pichon, Xavier and Sbarrato, Thomas and Roobol, Anne and Roobol, Jo and Vito, Davide and Bushell, Martin and von der Haar, Tobias and Smales, Christopher Mark and Mallucci, Giovanna R. and Willis, Anne E. (2017) RTN3 Is a Novel Cold-Induced Protein and Mediates Neuroprotective Effects of RBM3. Current Biology, 27 (5). pp. 638-650. ISSN 0960-9822. (doi:https://doi.org/10.1016/j.cub.2017.01.047) (Full text available)

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Saintas, Emily and Abrahams, Liam and Ahmed, Gulshan T and Ajakaiye, Anu-Oluwa M and AlHumaidi, Abdulaziz S H A M and Ashmore-Harris, Candice and Clark, Iain and Dura, Usha K and Fixmer, Carine N and Ike-Morris, Chinedu and Mato Prado, Mireia and Mccullough, Danielle and Mishra, Shishir and Schoeler, Katia M U and Timur, Husne and Williamson, Maxwell D C and Alatsatianos, Markella and Bahsoun, Basma and Blackburn, Edith and Hogwood, Catherine E.M. and Lithgow, Pamela E and Rowe, Michelle L. and Yiangou, Lyto and Rothweiler, Florian and Cinatl jr, Jindric and Zehner, Richard and Baines, Anthony J. and Garrett, Michelle D. and Gourlay, Campbell W. and Griffin, Darren K. and Gullick, William J. and Hargreaves, Emma and Howard, Mark J. and Lloyd, Daniel R. and Rossman, Jeremy S. and Smales, Christopher Mark and Tsaousis, Anastasios D. and von der Haar, Tobias and Wass, Mark N. and Michaelis, Martin (2017) Acquired resistance to oxaliplatin is not directly associated with increased resistance to DNA damage in SK-N-ASrOXALI4000, a newly established oxaliplatin-resistant sub-line of the neuroblastoma cell line SK-N-AS. PLoS ONE, 12 (2). e0172140. ISSN 1932-6203. (doi:https://doi.org/10.1371/journal.pone.0172140) (Full text available)

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Feary, M. and Racher, A.J. and Young, R.J. and Smales, Christopher Mark (2016) Methionine sulfoximine supplementation enhances productivity in GS-CHOK1SV cell lines through glutathione biosynthesis. Biotechnology Progress, 33 (1). pp. 17-25. ISSN 8756-7938. E-ISSN 1520-6033. (doi:https://doi.org/10.1002/btpr.2372) (Full text available)

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Josse, L. and Xie, J. and Proud, C. G. and Smales, Christopher Mark (2016) mTORC1 signalling and eIF4E/4E-BP1 translation initiation factor stoichiometry influence recombinant protein productivity from GS-CHOK1 cells. Biochemical Journal, 473 (24). pp. 4651-4664. ISSN 0264-6021. (doi:https://doi.org/10.1042/BCJ20160845) (Full text available)

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Williams, Marc J. and Sánchez, Enrique and Aluri, Esther Rani and Douglas, Fraser J. and MacLaren, Donald A. and Collins, Oonagh M. and Cussen, Edmund J. and Budge, James D. and Sanders, Lara C. and Michaelis, Martin and Smales, Christopher Mark and Cinatl, Jindrich and Lorrio, Silvia and Krueger, Dirk and de Rosales, Rafael T. M. and Corr, Serena A. (2016) Microwave-assisted synthesis of highly crystalline, multifunctional iron oxide nanocomposites for imaging applications. RSC Advances, 6 (87). pp. 83520-83528. ISSN 2046-2069. (doi:https://doi.org/10.1039/c6ra11819d) (Full text available)

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Lintern, Katherine and Pathak, Mili and Smales, Christopher Mark and Howland, Kevin and Rathore, Anurag and Bracewell, Daniel G. (2016) Residual on column host cell protein analysis during lifetime studies of protein A chromatography. Journal of Chromatography A, 1461 . pp. 70-77. ISSN 0021-9673. (doi:https://doi.org/10.1016/j.chroma.2016.07.055) (Full text available)

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Chiverton, Lesley M. and Evans, Caroline and Pandhal, Jagroop and Landels, Andrew R. and Rees, Byron J. and Levison, Peter R. and Wright, Phillip C. and Smales, Christopher Mark (2016) Quantitative definition and monitoring of the host cell protein proteome using iTRAQ: a study of an industrial mAb producing CHO-S cell line. Biotechnology Journal, 11 (8). pp. 1014-1024. ISSN 1860-6768. (doi:https://doi.org/10.1002/biot.201500550) (Full text available)

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Vasilev, Nikolay and Smales, Christopher Mark and Schillberg, Stefan and Fischer, Rainer and Schiermeyer, Andreas (2016) Developments in the production of mucosal antibodies in plants. Biotechnology Advances, 34 (2). pp. 77-87. ISSN 0734-9750. (doi:https://doi.org/10.1016/j.biotechadv.2015.11.002) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Gourbatsi, Evdoxia and Povey, Jane F. and Uddin, Shahid and Smales, Christopher Mark (2016) Biotherapeutic protein formulation variables influence protein integrity and can promote post-translational modifications as shown using chicken egg white lysozyme as a model system. Biotechnology Letters, 38 (4). pp. 589-596. ISSN 0141-5492. E-ISSN 1573-6776. (doi:https://doi.org/10.1007/s10529-015-2014-y) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Knight, John R.P. and Bastide, Amandine and Peretti, Diego and Roobol, Anne and Roobol, Joanne and Mallucci, Giovanna R. and Smales, Christopher Mark and Willis, Anne E. (2016) Cooling-induced SUMOylation of EXOSC10 down-regulates ribosome biogenesis. RNA, 22 (4). pp. 623-635. ISSN 1355-8382. E-ISSN 1469-9001. (doi:https://doi.org/10.1261/rna.054411.115) (Full text available)

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Mead, Emma J and Masterton, Rosalyn J. and Feary, Marc and Obrezanova, Olga and Zhang, Lin and Young, Robert and Smales, Christopher Mark (2015) Biological Insights into the Expression of Translation Initiation Factors from Recombinant CHOK1SV Cell Lines and their Relationship to Enhanced Productivity. Biochemical Journal, 472 (3). pp. 261-273. ISSN 0264-6021. E-ISSN 1470-8728. (doi:https://doi.org/10.1042/BJ20150928) (Full text available)

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Bracewell, Daniel G. and Francis, Richard and Smales, Christopher Mark (2015) The future of host cell protein (HCP) identification during process development and manufacturing linked to a risk-based management for their control. Biotechnology and Bioengineering, 112 (9). pp. 1727-1737. ISSN 0006-3592. (doi:https://doi.org/10.1002/bit.25628) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Tunjung, Woro Anindito Sri and Cinatl, Jindrich and Michaelis, Martin and Smales, Christopher Mark (2015) Anti-Cancer Effect of Kaffir Lime (Citrus Hystrix DC) Leaf Extract in Cervical Cancer and Neuroblastoma Cell Lines. Procedia Chemistry, 14 . pp. 465-468. ISSN 1876-6196. (doi:https://doi.org/10.1016/j.proche.2015.03.062) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Knight, John and Bastide, Amandine and Roobol, Anne and Roobol, Joanne and Jackson, Thomas and Utami, Wahyu and Barrett, David and Smales, Christopher Mark and Willis, A. (2015) Eukaryotic elongation factor 2 kinase regulates the cold stress response by slowing translation elongation. Biochemical Journal, 465 . pp. 227-238. ISSN 0264-6021. (doi:https://doi.org/10.1042/BJ20141014) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Rooney, Barrie C. and Piening, Turid and Büscher, Philippe and Rogé, Stijn and Smales, Christopher Mark (2015) Expression of Trypanosoma brucei gambiense Antigens in Leishmania tarentolae. Potential for Use in Rapid Serodiagnostic Tests (RDTs). PLOS Neglected Tropical Diseases, 9 (12). e0004271. ISSN 1935-2735. E-ISSN 1935-2735. (doi:https://doi.org/10.1371/journal.pntd.0004271) (Full text available)

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Roobol, Anne and Roobol, Joanne and Bastide, Amandine and Knight, John and Willis, Anne and Smales, Christopher Mark (2015) p58(IPK) is an Inhibitor of the eIF2? Kinase GCN2 and its Localisation and Expression Underpin Protein Synthesis and ER Processing Capacity. Biochemical Journal, 465 . pp. 213-225. ISSN 0264-6021. (doi:https://doi.org/10.1042/BJ20140852) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Hogwood, Catherine E.M. and Ahmad, Shahina S. and Tarrant, Richard D. and Bracewell, Daniel G. and Smales, Christopher Mark (2015) An ultra scale-down approach identifies host cell protein differences across a panel of mAb producing CHO cell line variants. Biotechnology Journal, 11 (3). pp. 415-424. ISSN 1860-6768. E-ISSN 1860-7314. (doi:https://doi.org/10.1002/biot.201500010) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Povey, Jane F. and O’Malley, Christopher J. and Root, Tracy and Martin, Elaine B. and Montague, Gary A. and Feary, Marc and Trim, Carol M. and Lang, Dietmar A. and Alldread, Richard and Racher, Andrew J. and Smales, Christopher Mark (2014) Rapid high-throughput characterisation, classification and selection of recombinant mammalian cell line phenotypes using intact cell MALDI-ToF mass spectrometry fingerprinting and PLS-DA modelling. Journal of Biotechnology, 184 . pp. 84-93. ISSN 0168-1656. (doi:https://doi.org/10.1016/j.jbiotec.2014.04.028) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Hogwood, Catherine EM and Bracewell, Daniel G and Smales, Christopher Mark (2014) Measurement and control of host cell proteins (HCPs) in CHO cell bioprocesses. Current Opinion in Biotechnology, 30 . pp. 153-160. ISSN 0958-1669. (doi:https://doi.org/10.1016/j.copbio.2014.06.017) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Ashton, Lorna and Hogwood, Catherine E.M. and Tait, Andrew S. and Kuligowski, Julia and Smales, Christopher Mark and Bracewell, Daniel G. and Dickson, Alan J. and Goodacre, Royston (2014) UV resonance Raman spectroscopy: a process analytical tool for host cell DNA and RNA dynamics in mammalian cell lines. Journal of Chemical Technology and Biotechnology, 90 (2). pp. 237-243. ISSN 0268-2575. (doi:https://doi.org/10.1002/jctb.4420) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Roobol, Anne and Roobol, Joanne and Carden, Martin J. and Smith, Matthew E. and Hershey, John W. B. and Bastide, Amandine and Knight, John R. P. and Willis, Anne E. and Smales, Christopher Mark (2014) The chaperonin CCT interacts with and mediates the correct folding and activity of three subunits of translation initiation factor eIF3: b, i and h. Biochemical Journal, 458 (2). pp. 213-224. ISSN 0264-6021. (doi:https://doi.org/10.1042/BJ20130979) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Masterton, Rosalyn J. and Smales, Christopher Mark (2014) The impact of process temperature on mammalian cell lines and the implications for the production of recombinant proteins in CHO cells. Pharmaceutical Bioprocessing, 2 (1). pp. 49-61. ISSN 2048-9145. (doi:https://doi.org/10.4155/pbp.14.3) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Al-Fageeh, Mohamed B. and Smales, Christopher Mark (2013) Alternative Promoters Regulate Cold Inducible RNA-Binding (CIRP) Gene Expression and Enhance Transgene Expression in Mammalian Cells. Molecular Biotechnology, 54 (2). pp. 238-249. ISSN 1073-6085. (doi:https://doi.org/10.1007/s12033-013-9649-5) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Hogwood, Catherine EM and Bracewell, Daniel G and Smales, Christopher Mark (2013) Host cell protein dynamics in recombinant CHO cells. Bioengineered, 4 (5). pp. 288-291. ISSN 2165-5979. (doi:https://doi.org/10.4161/bioe.23382) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Bracewell, Daniel G. and Smales, Christopher Mark (2013) The challenges of product- and process-related impurities to an evolving biopharmaceutical industry. Bioanalysis, 5 (2). pp. 123-126. ISSN 1757-6180. (doi:https://doi.org/10.4155/bio.12.314) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Hogwood, Catherine E.M. and Tait, Andrew S. and Koloteva-Levine, Nadejda and Bracewell, Daniel G. and Smales, Christopher Mark (2013) The dynamics of the CHO host cell protein profile during clarification and protein A capture in a platform antibody purification process. Biotechnology and Bioengineering, 110 (1). pp. 240-251. ISSN 0006-3592. (doi:https://doi.org/10.1002/bit.24607) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Wagstaff, Jane L. and Masterton, Rosalyn J. and Povey, Jane F. and Smales, Christopher Mark and Howard, Mark J. (2013) 1H NMR Spectroscopy Profiling of Metabolic Reprogramming of Chinese Hamster Ovary Cells upon a Temperature Shift during Culture. PLoS ONE, 8 (N/A). e77195-e77195. ISSN 1932-6203. (Full text available)

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Peters, Shirley J. and Smales, Christopher Mark and Henry, Alistair J. and Stephens, Paul E. and West, Shauna and Humphreys, David P. (2012) Engineering an Improved IgG4 Molecule with Reduced Disulfide Bond Heterogeneity and Increased Fab Domain Thermal Stability. Journal of Biological Chemistry, 287 (29). pp. 24525-24533. ISSN 0021-9258. (doi:https://doi.org/10.1074/jbc.M112.369744) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Mead, Emma J and Chiverton, Lesley M. and Spurgeon, Sarah K. and Martin, Elaine B. and Montague, Gary A. and Smales, Christopher Mark and von der Haar, Tobias (2012) Experimental and In Silico Modelling Analyses of the Gene Expression Pathway for Recombinant Antibody and By-Product Production in NS0 Cell Lines. PLoS ONE, 7 (10). e47422. ISSN 1932-6203. (doi:https://doi.org/10.1371/journal.pone.0047422) (Full text available)

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Tait, Andrew S. and Hogwood, Catherine E.M. and Smales, Christopher Mark and Bracewell, Daniel G. (2011) Host cell protein dynamics in the supernatant of a mAb producing CHO cell line. Biotechnology and Bioengineering, 109 (4). pp. 971-982. ISSN 0006-3592. (doi:https://doi.org/10.1002/bit.24383) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Kotov, Nikolay V. and Bates, Declan G. and Gizatullina, Antonina N. and Gilaziev, Bulat and Khairullin, Rustem N and Chen, Michael ZQ and Drozdov, Ignat and Umezawa, Yoshinori and Hundhausen, Christian and Aleksandrov, Alexey and Yan, Xinggang and Spurgeon, Sarah K. and Smales, Christopher Mark and Valeyev, Najl V. (2011) Computational modelling elucidates the mechanism of ciliary regulation in health and disease. BMC Systems Biology, 5 . p. 143. ISSN 1752-0509. (doi:https://doi.org/10.1186/1752-0509-5-143) (Full text available)

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Roobol, Anne and Roobol, Joanne and Carden, Martin J. and Bastide, Amandine and Willis, Anne E. and Dunn, Warwick B. and Goodacre, Royston and Smales, Christopher Mark (2011) ATR (ataxia telangiectasia mutated- and Rad3-related kinase) is activated by mild hypothermia in mammalian cells and subsequently activates p53. Biochemical Journal, 435 (2). pp. 499-508. ISSN 0264-6021. (doi:https://doi.org/10.1042/BJ20101303) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Hayes, Nandini V. L. and Jossé, Lyne and Smales, Christopher Mark and Carden, Martin J. (2011) Modulation of Phosducin-Like Protein 3 (PhLP3) Levels Promotes Cytoskeletal Remodelling in a MAPK and RhoA-Dependent Manner. PLoS ONE, 6 (12). e28271. ISSN 1932-6203. (doi:https://doi.org/10.1371/journal.pone.0028271) (Full text available)

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Reid, C.Q. and Tait, Andrew S. and Baldascini, H. and Mohindra, A. and Racher, Andrew J. and Bilsborough, S. and Smales, Christopher Mark and Hoare, Michael (2010) Rapid whole monoclonal antibody analysis by mass spectrometry: An Ultra scale-down study of the effect of harvesting by centrifugation on the post-translational modification profile. Biotechnology and Bioengineering, 107 (1). pp. 85-95. ISSN 0006-3592. (doi:https://doi.org/10.1002/bit.22790) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Jossé, Lyne and Smales, Christopher Mark and Tuite, Mick F. (2010) Transient expression of human TorsinA enhances secretion of two functionally distinct proteins in cultured Chinese hamster ovary (CHO) cells. Biotechnology and Bioengineering, 105 (3). pp. 556-566. ISSN 0006-3592. (doi:https://doi.org/10.1002/bit.22572) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Masterton, Rosalyn J. and Roobol, Anne and Al-Fageeh, Mohamed B. and Carden, Martin J. and Smales, Christopher Mark (2010) Post-translational events of a model reporter protein proceed with higher fidelity and accuracy upon mild hypothermic culturing of Chinese hamster ovary cells. Biotechnology and Bioengineering, 105 (1). pp. 215-220. ISSN 0006-3592. (doi:https://doi.org/10.1002/bit.22533) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Povey, Jane F. and Perez-Moral, Natalia and Noel, Timothy R. and Parker, Roger and Howard, Mark J. and Smales, Christopher Mark (2009) Investigating variables and mechanisms that influence protein integrity in low water content amorphous carbohydrate matrices. Biotechnology Progress, 25 (5). pp. 1217-27. ISSN 8756-7938. (doi:https://doi.org/10.1002/btpr.207) (Full text available)

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Mead, Emma J and Chiverton, Lesley M. and Smales, Christopher Mark and von der Haar, Tobias (2009) Identification of the limitations on recombinant gene expression in CHO cell lines with varying luciferase production rates. Biotechnology and Bioengineering, 102 (6). pp. 1593-1602. ISSN 0006-3592. (doi:https://doi.org/10.1002/bit.22201) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Roobol, Anne and Carden, Martin J. and Newsam, Ray J. and Smales, Christopher Mark (2009) Biochemical insights into the mechanisms central to the response of mammalian cells to cold stress and subsequent rewarming. FEBS Journal, 276 (1). pp. 286-302. ISSN 1742-464X. (doi:https://doi.org/10.1111/j.1742-4658.2008.06781.x) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Al-Fageeh, Mohamed B. and Smales, Christopher Mark (2009) Cold-inducible RNA binding protein (CIRP) expression is modulated by alternative mRNAs. RNA, 15 (6). pp. 1164-1176. ISSN 1355-8382. (doi:https://doi.org/10.1261/rna.1179109) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Hayes, Nandini V. L. and Smales, Christopher Mark and Klappa, Peter (2009) Protein disulfide isomerase does not control recombinant IgG4 productivity in mammalian cell lines. Biotechnology and Bioengineering, 105 (4). pp. 770-779. ISSN 0006-3592. (doi:https://doi.org/10.1002/bit.22587) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Smales, Christopher Mark (2008) Proceedings from the 8th PEACe Conference on Protein Expression in Animal Cells, Angra Dos Reis, Brazil, September 16-20, 2007. Molecular Biotechnology, 39 (2). p. 87. ISSN 1073-6085. (doi:https://doi.org/10.1007/s12033-008-9053-8) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Parsons, Joshua B. and Dinesh, Sriramulu D. and Deery, Evelyne and Leech, Helen K. and Brindley, Amanda A. and Heldt, Dana and Frank, Stefanie and Smales, Christopher Mark and Lunsdorf, Heinrich and Rambach, Alain and Gass, Mhairi H. and Bleloch, Andrew and McClean, Kirsty J. and Munro, Andrew W. and Rigby, Stephen E. J. and Warren, Martin J. and Prentice, Michael B. (2008) Biochemical and Structural Insights into Bacterial Organelle Form and Biogenesis. Journal of Biological Chemistry, 283 (21). pp. 14366-14375. ISSN 0021-9258. (doi:https://doi.org/10.1074/jbc.M709214200) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

Marchant, Rosalyn J. and Al-Fageeh, Mohamed B. and Underhill, Michele F. and Racher, Andrew J. and Smales, Christopher Mark (2008) Metabolic rates, growth phase, and mRNA levels influence cell-specific antibody production levels from in vitro-cultured mammalian cells at sub-physiological temperatures. Molecular Biotechnology, 39 (1). pp. 69-77. ISSN 1073-6085. (doi:https://doi.org/10.1007/s12033-008-9032-0) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Povey, Jane F. and Howard, Mark J. and Williamson, Richard A. and Smales, Christopher Mark (2008) The effect of peptide glycation on protein secondary structure. Journal of Structural Biology, 161 (2). pp. 151-161. ISSN 1047-8477. (doi:https://doi.org/10.1016/j.jsb.2007.10.004) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

Underhill, Michele F. and Smales, Christopher Mark (2007) The cold-shock response in mammalian cells: investigating the HeLa cell cold-shock proteome. Cytotechnology, 53 (1-3). pp. 47-53. ISSN 0920-9069. (doi:https://doi.org/10.1007/s10616-007-9048-5) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Povey, Jane F. and Smales, Christopher Mark and Hassard, S.J. and Howard, Mark J. (2007) Comparison of the effects of 2,2,2-trifluoroethanol on peptide and protein structure and function. Journal of Structural Biology, 157 (2). pp. 329-338. ISSN 1047-8477. (doi:https://doi.org/10.1016/j.jsb.2006.07.008) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

Underhill, Michele F. and Smales, Christopher Mark and Naylor, Louise H. and Birch, John R. and James, David C. (2007) Transient Gene Expression Levels from Multigene Expression Vectors. Biotechnology Progress, 23 (2). pp. 435-443. ISSN 8756-7938. (doi:https://doi.org/10.1021/bp060225z) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Underhill, Michele F. and Marchant, Rosalyn J. and Carden, Martin J. and James, David C. and Smales, Christopher Mark (2006) On the effect of transient expression of mutated eIF2 alpha and eIF4E eukaryotic translation initiation factors on reporter gene expression in mammalian cells upon cold-shock. Molecular Biotechnology, 34 (2). pp. 141-149. ISSN 1073-6085. (doi:https://doi.org/10.1385/MB:34:2:141) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Dinnis, Diane M. and Stansfield, Scott H. and Schlatter, Stefan and Smales, Christopher Mark and Alete, Daniel E. and Birch, John R. and Racher, Andrew J. and Marshal, Carol T. and Nielsen, Lars K. and James, David C. (2006) Functional proteomic analysis of GS-NS0 murine myeloma cell lines with varying recombinant monoclonal antibody production rate. Biotechnology and Bioengineering, 94 (5). pp. 830-841. ISSN 0006-3592. (doi:https://doi.org/10.1002/bit.20899) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Weeks, Mark E. and von Caron, G.N. and James, David C. and Smales, Christopher Mark and Robinson, Gary K. (2006) Monitoring changes in nisin susceptibility of Listeria monocytogenes Scott A as an indicator of growth phase using FACS. Journal of Microbiological Methods, 66 (1). pp. 43-55. ISSN 0167-7012. (doi:https://doi.org/10.1016/j.mimet.2005.10.008) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Ahmad, Norhaiza and Zhang, Jian and Brown, Philip J. and James, David C. and Birch, John R. and Racher, Andrew J. and Smales, Christopher Mark (2006) On the statistical analysis of the GS-NS0 cell proteome: Imputation, clustering and variability testing. Biochimica Et Biophysica Acta-Proteins and Proteomics, 1764 (7). pp. 1179-1187. ISSN 1570-9639. (doi:https://doi.org/10.1016/j.bbapap.2006.05.002) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Al-Fageeh, Mohamed B. and Marchant, Rosalyn J. and Carden, Martin J. and Smales, Christopher Mark (2006) The cold-shock response in cultured mammalian cells: Harnessing the response for the improvement of recombinant protein production. Biotechnology and Bioengineering, 93 (5). pp. 829-835. ISSN 0006-3592. (doi:https://doi.org/10.1002/bit.20789) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Al-Fageeh, Mohamed B. and Smales, Christopher Mark (2006) Control and regulation of the cellular responses to cold shock: the responses in yeast and mammalian systems. Biochemical Journal, 397 . pp. 247-259. ISSN 0264-6021. (doi:https://doi.org/10.1042/BJ20060166) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Gourbatsi, Evdoxia and Al-Fageeh, Mohamed B. and Marchant, Rosalyn J. and Scott, Sarah J. and Underhill, Michele F. and Smales, Christopher Mark (2006) Noncovalently linked nuclear localization peptides for enhanced calcium phosphate transfection. Molecular Biotechnology, 33 (1). pp. 1-11. ISSN 1073-6085. (doi:https://doi.org/10.1385/MB:33:1:1) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Alete, Daniel E. and Racher, Andrew J. and Birch, John R. and Stansfield, Scott H. and James, David C. and Smales, Christopher Mark (2005) Proteomic analysis of enriched microsomal fractions from GS-NS0 murine myeloma cells with varying secreted recombinant monoclonal antibody productivities. Proteomics, 5 (18). pp. 4689-4704. ISSN 1615-9853. (doi:https://doi.org/10.1002/pmic.200500019) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Underhill, Michele F. and Birch, John R. and Smales, Christopher Mark and Naylor, Louise H. (2005) eIF2 alpha phosphorylation, stress perception, and the shutdown of global protein synthesis in cultured CHO cells. Biotechnology and Bioengineering, 89 (7). pp. 805-814. ISSN 0006-3592. (doi:https://doi.org/10.1002/bit.20403) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Howard, Mark J. and Smales, Christopher Mark (2005) NMR analysis of synthetic human serum albumin alpha-helix 28 identifies structural distortion upon amadori modification. Journal of Biological Chemistry, 280 (24). pp. 22582-22589. ISSN 0021-9258. (doi:https://doi.org/10.1074/jbc.M501480200) (Full text available)

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Smales, Christopher Mark and Dinnis, Diane M. and Stansfield, Scott H. and Alete, Daniel E. and Sage, E.A. and Birch, John R. and Racher, Andrew J. and Marshall, Carol T. and James, David C. (2004) Comparative proteomic analysis of GS-NSO murine myeloma cell lines with varying recombinant monoclonal antibody production rate. Biotechnology and Bioengineering, 88 (4). pp. 474-488. ISSN 0006-3592. (doi:https://doi.org/10.1002/bit.20272) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

Weeks, Mark E. and James, David C. and Robinson, Gary K. and Smales, Christopher Mark (2004) Global changes in gene expression observed at the transition from growth to stationary phase in Listeria monocytogenes ScottA batch culture. Proteomics, 4 (1). pp. 123-135. ISSN 1615-9853. (doi:https://doi.org/10.1002/pmic.200300527) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

Cooke, D.G. and Flight, S. and Smales, Christopher Mark and Blackwell, Leonard F. (2003) Use of defined estrone glucuronide-hen egg white lysozyme conjugates as signal generators in homogeneous enzyme immunoassays for urinary estrone glucuronide. Journal of Immunoassay & Immunochemistry, 24 (2). pp. 147-172. ISSN 1532-1819. (doi:https://doi.org/10.1081/IAS-120020082) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Smales, Christopher Mark and Birch, John R. and Racher, Andrew J. and Marshall, Carol T. and James, David C. (2003) Evaluation of individual protein errors in silver-stained two-dimensional gels. Biochemical and Biophysical Research Communications, 306 (4). pp. 1050-1055. ISSN 0006-291X. (doi:https://doi.org/10.1016/S0006-291X(03)01115-X) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Smales, Christopher Mark and Blackwell, Leonard F. (2003) Lysozyme conjugate immune complex formation and the effects on substrate hydrolysis. Biochemical and Biophysical Research Communications, 304 (4). pp. 818-824. ISSN 0006-291X. (doi:https://doi.org/10.1016/S0006-291X(03)00684-3) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Smales, Christopher Mark and Pepper, Duncan S. and James, David C. (2002) Protein modification during anti-viral heat-treatment bioprocessing of factor VIII concentrates, factor IX concentrates, and model proteins in the presence of sucrose. Biotechnology and Bioengineering, 77 (1). pp. 37-48. ISSN 0006-3592. (doi:https://doi.org/10.1002/bit.10161) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

Smales, Christopher Mark and Blackwell, Leonard F. (2002) Purification and characterization of lysozyme-pregnanediol glucuronide conjugates: the effect of the hapten and coupling reagent on the substitution level, sites of acylation and the consequences for the development of future immunoassays. Biotechnology and Applied Biochemistry, 36 (2). pp. 101-110. ISSN 0885-4513. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Smales, Christopher Mark and Pepper, Duncan S. and James, David C. (2001) Protein Modifications during Antiviral Heat Bioprocessing and Subsequent Storage. Biotechnology Progress, 17 (5). pp. 974-978. ISSN 8756-7938. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Smales, Christopher Mark and Pepper, Duncan S. and James, David C. (2001) Evaluation of Protein Modification during Anti-Viral Heat Bioprocessing by Electrospray Ionization Mass Spectrometry. Rapid Communications in Mass Spectrometry, 15 (5). pp. 351-356. ISSN 0951-4198. (doi:https://doi.org/10.1002/rcm.232) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Davis, Paul J. and Smales, Christopher Mark and James, David C. (2001) How can Thermal Processing Modify the Antigenicity of Proteins? Allergy, 56 (s67). pp. 56-60. ISSN 0105-4538. (doi:https://doi.org/10.1034/j.1398-9995.2001.00918.x) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Smales, Christopher Mark and Pepper, Duncan S. and James, David C. (2000) Mechanisms of protein modification during model anti-viral heat-treatment bioprocessing of beta-lactoglobulin variant A in the presence of sucrose. Biotechnology and Applied Biochemistry, 32 (2). pp. 109-119. ISSN 0885-4513. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Smales, Christopher Mark and Pepper, Duncan S. and James, David C. (2000) Protein modification during antiviral heat bioprocessing. Biotechnology and Bioengineering, 67 (2). pp. 177-188. ISSN 0006-3592. (doi:https://doi.org/10.1002/(SICI)1097-0290(20000120)67:2<177::AID-BIT7>3.0.CO;2-3) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Smales, Christopher Mark and Moore, Christopher H. and Blackwell, Leonard F. (1999) Characterization of lysozyme-estrone glucuronide conjugates. The effect of the coupling reagent on the substitution level and sites of acylation. Bioconjugate Chemistry, 10 (4). pp. 693-700. ISSN 1043-1802. (doi:https://doi.org/10.1021/bc9900441) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Smales, Christopher Mark and Elgar, David F. and Moore, Christopher H. and Blackwell, Leonard F. (1998) Acid-polyacrylamide gel electrophoresis of lysozyme-estrone glucuronide conjugates. Bioconjugate Chemistry, 9 (6). pp. 838-841. ISSN 1043-1802. (doi:https://doi.org/10.1021/bc9701914) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Smales, Christopher Mark and Blackwell, Leonard F. and Waters, J.M. and Burrell, A.K. (1997) An estrone-glucuronide conjugate. Acta Crystallographica Section C-Crystal Structure Communications, 53 (part 8). pp. 1082-1084. ISSN 0108-2701. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Smales, Christopher Mark and Cooke, D.G. and Blackwell, Leonard F. (1994) Use of ion-exchange and hydrophobic-interaction chromatography for the rapid purification of lysozyme-estrone glucuronide conjugates. Journal of Chromatography B-Biomedical Applications, 662 (1). pp. 3-14. ISSN 0378-4347. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Book section

Jossé, Lyne and Smales, Christopher Mark and Tuite, Mick F. (2012) Engineering the Chaperone Network of CHO Cells for Optimal Recombinant Protein Production and Authenticity. In: Recombinant Gene Expression. Methods in Molecular Biology, 824 . Springer New York, pp. 595-608. ISBN 978-1-61779-433-9. (doi:https://doi.org/10.1007/978-1-61779-433-9_32) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Mead, Emma J and Smales, Christopher Mark (2011) mRNA Translation and Recombinant Gene Expression from Mammalian Cell Expression Systems. In: Comprehensive Biotechnology. Elsevier, pp. 403-409. ISBN 978-0-08-088504-9. (doi:https://doi.org/10.1016/B978-0-08-088504-9.00043-X) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Scott, Sarah J. and Marchant, Rosalyn J. and Al-Fageeh, Mohamed B. and Underhill, Michele F. and Smales, Christopher Mark (2006) The molecular response(s) during cellular adaptation to, and recovery from, sub-physiological temperatures. In: Al-Rubeai, Mohamed and Fussenegger, Martin M., eds. Systems Biology. Cell Engineering (5). Springer, Netherlands, pp. 185-212. ISBN 978-1-4020-5251-4. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Alete, Daniel E. and Racher, R.J. and Birch, John R. and James, David C. and Smales, Christopher Mark (2005) The functional competence of animal cells: Analysis of the secretory pathway. In: Godia, Francesc and Fussenegger, Martin M., eds. Animal Cell Technology Meets Genomics. ESACT Proceedings, 2 . Springer, Dordrecht, pp. 71-74. ISBN 978-1-4020-2791-8. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Thesis

Mozzanino, Théo (2018) Engineering of the Secretory Pathway of CHO Cells for Recombinant Protein Production: Manipulation of SNARE Proteins. Doctor of Philosophy (PhD) thesis, University of Kent,. (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

Binge, Alexandra (2018) Development of Endogenous and Synthetic CHO Promoter Expression Systems for Recombinant Protein Production. Doctor of Philosophy (PhD) thesis, University of Kent,. (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

Godfrey, Charlotte (2017) Investigation of Translational Reprogramming during Transient and Stable Expression of Monoclonal Antibodies in Chinese Hamster Ovary Cells. Doctor of Philosophy (PhD) thesis, University of Kent,. (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

Patel, Tulshi (2017) Manipulation and Exploitation of MicroRNAs for Enhanced Recombinant Protein Production in Chinese Hamster Ovary Cells. Doctor of Philosophy (PhD) thesis, University of Kent,. (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

Hutton, Rachael (2015) Investigating the Role of TOR Signalling, and the Link between Actin Binding Protein Cofilin and Mitochondrial Function and their Effects on Recombinant Protein Production. Doctor of Philosophy (PhD) thesis, University of Kent. (Full text available)

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Davies, Stephanie Ann (2014) Determination of Bioprocessing Variables that Influence Particle and Aggregation Formation of Therapeutic IgGs. Doctor of Philosophy (PhD) thesis, University of Kent,. (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

Dean, Andrew (2014) Investigating the Restraints Upon Recombinant Protein Production in Mammalian Cells via the Manipulation of eEF2, eEF2K and mTOR. Doctor of Philosophy (PhD) thesis, University of Kent,. (Full text available)

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Creigh-Pulatmen, Tilbe (2014) Structural and functional characterisation of the cold-inducible RNA-binding protein CIRP and its application to enhanced recombinant protein production. Doctor of Philosophy (PhD) thesis, University of Kent,. (Full text available)

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Edited book

Smales, Christopher Mark and James, David C., eds. (2005) Characterization of therapeutic proteins by membrane and in-gel tryptic digestion. Methods in Molecular Biology, 308 . Humana Press, 482 pp. ISBN 978-1-58829-390-9. (doi:https://doi.org/10.1385/1-59259-922-2:375) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)