TORC2 dependent phosphorylation 1 modulates calcium regulation of fission yeast myosin

Baker, Karen and Gyamfi, I. and Mashanov, Gregory I and Molloy, Justin E and Geeves, Michael A. and Mulvihill, Daniel P. (2018) TORC2 dependent phosphorylation 1 modulates calcium regulation of fission yeast myosin. EMBO Journal, . ISSN 0261-4189. E-ISSN 1460-2075. (Submitted) (Full text available)

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All cells have the ability to respond to changes in their environment. Signalling networks modulate cytoskeleton and membrane organisation to impact cell cycle progression, polarised cell growth and multicellular development according to the environmental setting. Using diverse in vitro, in vivo and single molecule techniques we have explored the role of myosin-1 signalling in regulating endocytosis during both mitotic and meiotic cell cycles. We have established that a conserved serine within the neck region of the sole fission yeast myosin-1 is phosphorylated in a TORC2 dependent manner to modulate myosin function. Myo1 neck phosphorylation brings about a change in the conformation of the neck region and modifies its interaction with calmodulins, Myo1 dynamics at endocytic foci, and promotes calcium dependent switching between different calmodulin light chains. These data provide insight into a novel mechanism by which myosin neck phosphorylation modulates acto44 myosin dynamics to control polarised cell growth in response to mitotic and meiotic cell-cycle progression and the cellular environment.

Item Type: Article
Uncontrolled keywords: pombe, endocytosis, calmodulin, Tor kinase
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Dan Mulvihill
Date Deposited: 19 Dec 2018 12:14 UTC
Last Modified: 07 Feb 2019 13:55 UTC
Resource URI: (The current URI for this page, for reference purposes)
Geeves, Michael A.:
Mulvihill, Daniel P.:
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