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Ultrathin and smooth poly(methyl methacrylate) (PMMA) films for label-free biomolecule detection with total internal reflection ellipsometry (TIRE)

Nam Cao, Hoai le, Gubala, Vladimir, Clancy, Eoin, Barry, Thomas, Smith, Terry J, Williams, David E. (2012) Ultrathin and smooth poly(methyl methacrylate) (PMMA) films for label-free biomolecule detection with total internal reflection ellipsometry (TIRE). Biosensors and Bioelectronics, 36 (1). pp. 250-256. ISSN 0956-5663. (doi:10.1016/j.bios.2012.04.032) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1016/j.bios.2012.04.032

Abstract

Ultrathin poly(methyl methacrylate) PMMA films were prepared on gold substrates by spin coating PMMA dissolved in toluene. By varying the concentration of PMMA, spin coating speed and curing condition, we obtained very smooth and ultrathin PMMA films. The PMMA films were transformed into highly reactive film containing carboxylic functionalities using UV/O3 irradiation. These films were shown to remain stable and reactive for at least one week. We then demonstrated the application of the UV/O3 treated PMMA films for the detection of microRNAs using a label-free detection method called total internal reflection ellipsometry (TIRE). A limit of detection of 10 pM was established. The technique proposed here is a simple and quick method for generating carboxylic functional films for label-free bioanalytical detection techniques.

Item Type: Article
DOI/Identification number: 10.1016/j.bios.2012.04.032
Additional information: The author made a substantial contribution to the conception and design of the study and to the organisation of the conduct of the study and to carrying out the study and to analysis and interpretation of the study data. The author helped draft the output and to critique the output for important intellectual content.; number of additional authors: 5;
Subjects: Q Science
R Medicine > RS Pharmacy and materia medica
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Stewart Brownrigg
Date Deposited: 07 Mar 2014 00:05 UTC
Last Modified: 29 May 2019 12:24 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/40396 (The current URI for this page, for reference purposes)
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