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Number of items: 50.

Article

Peters, S.J. and Smales, C.M. and Henry, A.J. and Stephens, P.E. and West, S. and Humphreys, D.P. (2012) Engineering an Improved IgG4 Molecule with Reduced Disulfide Bond Heterogeneity and Increased Fab Domain Thermal Stability. Journal of Biological Chemistry, 287 (29). pp. 24525-24533. ISSN 0021-9258. The full text of this publication is not available from this repository.

Jossé, Lyne and Smales, C.M. and Tuite, M.F. (2012) Engineering the Chaperone Network of CHO Cells for Optimal Recombinant Protein Production and Authenticity. Methods in Molecular Biology, 824 (6). pp. 595-608. ISSN 1064-3745. The full text of this publication is not available from this repository.

Tait, A.S. and Hogwood, C.E.M. and Smales, C.M. and Bracewell, D.G. (2011) Host cell protein dynamics in the supernatant of a mAb producing CHO cell line. Biotechnology and Bioengineering, 109 (4). pp. 971-982. ISSN 00063592. The full text of this publication is not available from this repository.

Kotov, N.V. and Bates, D.G. and Gizatullina, A.N. and Gilaziev, B. and Khairullin, R.N. and Chen, M.Z.Q. and Drozdov, I. and Umezawa, Y. and Hundhausen, C. and Aleksandrov, A. and Yan, X.G. and Spurgeon, S.K. and Smales, C.M. and Valeyev, Najl V. (2011) Computational modelling elucidates the mechanism of ciliary regulation in health and disease. BMC Systems Biology, 5 . p. 143. ISSN 1752-0509.

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Roobol, Anne and Roobol, Jo and Carden, M.J. and Bastide, Amandine and Willis, Anne E. and Dunn, Warwick B. and Goodacre, Royston and Smales, C.M. (2011) ATR (ataxia telangiectasia mutated- and Rad3-related kinase) is activated by mild hypothermia in mammalian cells and subsequently activates p53. Biochemical Journal, 435 (2). pp. 499-508. ISSN 0264-6021. The full text of this publication is not available from this repository.

Rao, J. and Hayes, N.V.L. and Jossé, Lyne and Smales, C.M. and Carden, M.J. (2011) Modulation of Phosducin-Like Protein 3 (PhLP3) Levels Promotes Cytoskeletal Remodelling in a MAPK and RhoA-Dependent Manner. PLoS ONE, 6 (12). e28271. ISSN 1932-6203.

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Reid, C.Q. and Tait, A. and Baldascini, H. and Mohindra, A. and Racher, A. and Bilsborough, S. and Smales, C.M. and Hoare, M. (2010) Rapid whole monoclonal antibody analysis by mass spectrometry: An Ultra scale-down study of the effect of harvesting by centrifugation on the post-translational modification profile. Biotechnology and Bioengineering, 107 (1). pp. 85-95. ISSN 0006-3592. The full text of this publication is not available from this repository.

Jossé, Lyne and Smales, C.M. and Tuite, M.F. (2010) Transient expression of human TorsinA enhances secretion of two functionally distinct proteins in cultured Chinese hamster ovary (CHO) cells. Biotechnology and Bioengineering, 105 (3). pp. 556-566. ISSN 0006-3592. The full text of this publication is not available from this repository.

Povey, J. and Perez-Moral, N. and Noel, T.R. and Parker, R. and Howard, M.J. and Smales, C.M. (2009) Investigating variables and mechanisms that influence protein integrity in low water content amorphous carbohydrate matrices. Biotechnol Prog, 25 (5). pp. 1217-27. ISSN 1520-6033.

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Mead, E.J. and Chiverton, L.M. and Smales, C.M. and von der Haar, T. (2009) Identification of the limitations on recombinant gene expression in CHO cell lines with varying luciferase production rates. Biotechnology and Bioengineering, 102 (6). pp. 1593-1602. ISSN 0006-3592 . The full text of this publication is not available from this repository.

Roobol, A. and Carden, M.J. and Newsam, R.J. and Smales, C.M. (2009) Biochemical insights into the mechanisms central to the response of mammalian cells to cold stress and subsequent rewarming. Febs Journal, 276 (1). pp. 286-302. ISSN 1742464x. The full text of this publication is not available from this repository.

Masterton, R.J. and Roobol, A. and Al-Fageeh, M.B. and Carden, M.J. and Smales, C.M. (2009) Post-translational events of a model reporter protein proceed with higher fidelity and accuracy upon mild hypothermic culturing of Chinese hamster ovary cells. Biotechnology and Bioengineering, Early View Online . ISSN 1097-02950 Online. The full text of this publication is not available from this repository.

Hayes, N.V.L. and Smales, C.M. and Klappa, P. (2009) Protein disulfide isomerase does not control recombinant IgG4 productivity in mammalian cell lines. Biotechnology and Bioengineering, 105 (4). pp. 770-779. ISSN 0006-3592. The full text of this publication is not available from this repository.

Smales, C.M. (2008) Proceedings from the 8th PEACe Conference on Protein Expression in Animal Cells, Angra Dos Reis, Brazil, September 16-20, 2007. Molecular Biotechnology, 39 (2). p. 87. ISSN 1073-6085. The full text of this publication is not available from this repository.

Parsons, J.B. and Dinesh, S.D. and Deery, E. and Leech, H.K. and Brindley, A.A. and Heldt, D. and Frank, S. and Smales, C.M. and Lunsdorf, H. and Rambach, A. and Gass, M.H. and Bleloch, A. and McClean, K.J. and Munro, A.W. and Rigby, S.E. and Warren, M.J. and Prentice, M.B. (2008) Biochemical and Structural Insights into Bacterial Organelle Form and Biogenesis. Journal of Biochemistry, 283 (21). pp. 14366-14375. ISSN 0021-9258 (Print). The full text of this publication is not available from this repository.

Marchant, R.J. and Al-Fageeh, M.B. and Underhill, M.F. and Racher, A.J. and Smales, C.M. (2008) Metabolic rates, growth phase, and mRNA levels influence cell-specific antibody production levels from in vitro-cultured mammalian cells at sub-physiological temperatures. Molecular Biotechnology, 39 (1). pp. 69-77. ISSN 1073-6085. The full text of this publication is not available from this repository.

Povey, J. and Howard, M.J. and Williamson, R.A. and Smales, C.M. (2008) The effect of peptide glycation on protein secondary structure. Journal of Structural Biology, 161 (2). pp. 151-161. ISSN 1047-8477. Access to this publication is restricted.

Underhill, M.F. and Smales, C.M. (2007) The cold-shock response in mammalian cells: investigating the HeLa cell cold-shock proteome. Cytotechnology, 53 (1-3). pp. 47-53. ISSN 0920-9069 . The full text of this publication is not available from this repository.

Povey, J. and Smales, C.M. and Hassard, S.J. and Howard, M.J. (2007) Comparison of the effects of 2,2,2-trifluoroethanol on peptide and protein structure and function. Journal of Structural Biology, 157 (2). pp. 329-338. ISSN 1047-8477. Access to this publication is restricted.

Underhill, M.F. and Smales, C.M. and Naylor, L.H. and Birch, J.R. and James, D.C. (2007) Transient Gene Expression Levels from Multigene Expression Vectors. Biotechnology Progress, 23 (2). pp. 435-443. ISSN 8756-7938 . The full text of this publication is not available from this repository.

Underhill, M.F. and Marchant, R.J. and Carden, M.J. and James, D.C. and Smales, C.M. (2006) On the effect of transient expression of mutated eIF2 alpha and eIF4E eukaryotic translation initiation factors on reporter gene expression in mammalian cells upon cold-shock. Molecular Biotechnology, 34 (2). pp. 141-149. ISSN 1073-6085. The full text of this publication is not available from this repository.

Dinnis, D.M. and Stansfield, S.H. and Schlatter, S. and Smales, C.M. and Alete, D.E. and Birch, J.R. and Racher, A.J. and Marshal, C.T. and Nielsen, L.K. and James, D.C. (2006) Functional proteomic analysis of GS-NS0 murine myeloma cell lines with varying recombinant monoclonal antibody production rate. Biotechnology and Bioengineering, 94 (5). pp. 830-841. ISSN 0006-3592. The full text of this publication is not available from this repository.

Weeks, M.E. and von Caron, G.N. and James, D.C. and Smales, C.M. and Robinson, G.K. (2006) Monitoring changes in nisin susceptibility of Listeria monocytogenes Scott A as an indicator of growth phase using FACS. Journal of Microbiological Methods, 66 (1). pp. 43-55. ISSN 0167-7012. The full text of this publication is not available from this repository.

Ahmad, N. and Zhang, J. and Brown, P.J. and James, D. and Birch, J.R. and Racher, A.J. and Smales, C.M. (2006) On the statistical analysis of the GS-NS0 cell proteome: Imputation, clustering and variability testing. Biochimica Et Biophysica Acta-Proteins and Proteomics, 1764 (7). pp. 1179-1187. ISSN 1570-9639. The full text of this publication is not available from this repository.

Al-Fageeh, M.B. and Marchant, R.J. and Carden, M.J. and Smales, C.M. (2006) The cold-shock response in cultured mammalian cells: Harnessing the response for the improvement of recombinant protein production. Biotechnology and Bioengineering, 93 (5). pp. 829-835. ISSN 0006-3592. The full text of this publication is not available from this repository.

Al-Fageeh, M.B. and Smales, C.M. (2006) Control and regulation of the cellular responses to cold shock: the responses in yeast and mammalian systems. Biochemical Journal, 397 . pp. 247-259. ISSN 0264-6021. The full text of this publication is not available from this repository.

Gourbatsi, E. and Al-Fageeh, M.B. and Marchant, R.J. and Scott, S.J. and Underhill, M.F. and Smales, C.M. (2006) Noncovalently linked nuclear localization peptides for enhanced calcium phosphate transfection. Molecular Biotechnology, 33 (1). pp. 1-11. ISSN 1073-6085. The full text of this publication is not available from this repository.

Alete, D.E. and Racher, A.J. and Birch, J.R. and Stansfield, S.H. and James, D.C. and Smales, C.M. (2005) Proteomic analysis of enriched microsomal fractions from GS-NS0 murine myeloma cells with varying secreted recombinant monoclonal antibody productivities. Proteomics, 5 (18). pp. 4689-4704. ISSN 1615-9853. The full text of this publication is not available from this repository.

Underhill, M.F. and Birch, J.R. and Smales, C.M. and Naylor, L.H. (2005) eIF2 alpha phosphorylation, stress perception, and the shutdown of global protein synthesis in cultured CHO cells. Biotechnology and Bioengineering, 89 (7). pp. 805-814. ISSN 0006-3592. The full text of this publication is not available from this repository.

Howard, M.J. and Smales, C.M. (2005) NMR analysis of synthetic human serum albumin alpha-helix 28 identifies structural distortion upon amadori modification. Journal of Biological Chemistry, 280 (24). pp. 22582-22589. ISSN 0021-9258.

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Smales, C.M. and Dinnis, D.M. and Stansfield, S.H. and Alete, D.E. and Sage, E.A. and Birch, J.R. and Racher, A.J. and Marshall, C.T. and James, D.C. (2004) Comparative proteomic analysis of GS-NSO murine myeloma cell lines with varying recombinant monoclonal antibody production rate. Biotechnology and Bioengineering, 88 (4). pp. 474-488. ISSN 0006-3592. Access to this publication is restricted.

Weeks, M.E. and James, D.C. and Robinson, G.K. and Smales, C.M. (2004) Global changes in gene expression observed at the transition from growth to stationary phase in Listeria monocytogenes ScottA batch culture. Proteomics, 4 (1). pp. 123-135. ISSN 1615-9853. Access to this publication is restricted.

Cooke, D.G. and Flight, S. and Smales, C.M. and Blackwell, L.F. (2003) Use of defined estrone glucuronide-hen egg white lysozyme conjugates as signal generators in homogeneous enzyme immunoassays for urinary estrone glucuronide. Journal of Immunoassay & Immunochemistry, 24 (2). pp. 147-172. ISSN 1532-1819. The full text of this publication is not available from this repository.

Smales, C.M. and Birch, J.R. and Racher, A.J. and Marshall, C.T. and James, D.C. (2003) Evaluation of individual protein errors in silver-stained two-dimensional gels. Biochemical and Biophysical Research Communications, 306 (4). pp. 1050-1055. ISSN 0006-291X. The full text of this publication is not available from this repository.

Smales, C.M. and Blackwell, L.F. (2003) Lysozyme conjugate immune complex formation and the effects on substrate hydrolysis. Biochemical and Biophysical Research Communications, 304 (4). pp. 818-824. ISSN 0006-291X. The full text of this publication is not available from this repository.

Smales, C.M. and Pepper, D.S. and James, D.C. (2002) Protein modification during anti-viral heat-treatment bioprocessing of factor VIII concentrates, factor IX concentrates, and model proteins in the presence of sucrose. Biotechnology and Bioengineering, 77 (1). pp. 37-48. ISSN 0006-3592. Access to this publication is restricted.

Smales, C.M. and Blackwell, L.F. (2002) Purification and characterization of lysozyme-pregnanediol glucuronide conjugates: the effect of the hapten and coupling reagent on the substitution level, sites of acylation and the consequences for the development of future immunoassays. Biotechnology and Applied Biochemistry, 36 (2). pp. 101-110. ISSN 0885-4513. The full text of this publication is not available from this repository.

Smales, C.M. and Pepper, D.S. and James, D.C. (2001) Protein Modifications during Antiviral Heat Bioprocessing and Subsequent Storage. Biotechnology Progress, 17 (5). pp. 974-978. ISSN 8756-7938 . The full text of this publication is not available from this repository.

Smales, C.M. and Pepper, D.S. and James, D.C. (2001) Evaluation of Protein Modification during Anti-Viral Heat Bioprocessing by Electrospray Ionization Mass Spectrometry. Rapid Communications in Mass Spectrometry, 15 (5). pp. 351-356. ISSN 0951-4198. The full text of this publication is not available from this repository.

Davis, P.J. and Smales, C.M. and James, D.C. (2001) How can Thermal Processing Modify the Antigenicity of Proteins? Allergy, 56 (s67). pp. 56-60. ISSN 0105-4538. The full text of this publication is not available from this repository.

Smales, C.M. and Pepper, D.S. and James, D.C. (2000) Mechanisms of protein modification during model anti-viral heat-treatment bioprocessing of beta-lactoglobulin variant A in the presence of sucrose. Biotechnology and Applied Biochemistry, 32 (2). pp. 109-119. ISSN 0885-4513. The full text of this publication is not available from this repository.

Smales, C.M. and Pepper, D.S. and James, D.C. (2000) Protein modification during antiviral heat bioprocessing. Biotechnology and Bioengineering, 67 (2). pp. 177-188. ISSN 0006-3592. The full text of this publication is not available from this repository.

Smales, C.M. and Moore, C.H. and Blackwell, L.F. (1999) Characterization of lysozyme-estrone glucuronide conjugates. The effect of the coupling reagent on the substitution level and sites of acylation. Bioconjugate Chemistry, 10 (4). pp. 693-700. ISSN 1043-1802. The full text of this publication is not available from this repository.

Smales, C.M. and Harding, D.R.K. (1999) A novel target synthesis laboratory for students. Journal of Chemical Education, 76 (11). pp. 1558-1560. ISSN 0021-9584. The full text of this publication is not available from this repository.

Smales, C.M. and Elgar, D.F. and Moore, C.H. and Blackwell, L.F. (1998) Acid-polyacrylamide gel electrophoresis of lysozyme-estrone glucuronide conjugates. Bioconjugate Chemistry, 9 (6). pp. 838-841. ISSN 1043-1802. The full text of this publication is not available from this repository.

Smales, C.M. and Blackwell, L.F. and Waters, J.M. and Burrell, A.K. (1997) An estrone-glucuronide conjugate. Acta Crystallographica Section C-Crystal Structure Communications, 53 (part 8). pp. 1082-1084. ISSN 0108-2701. The full text of this publication is not available from this repository.

Smales, C.M. and Cooke, D. and Blackwell, L.F. (1994) Use of ion-exchange and hydrophobic-interaction chromatography for the rapid purification of lysozyme-estrone glucuronide conjugates. Journal of Chromatography B-Biomedical Applications, 662 (1). pp. 3-14. ISSN 0378-4347. The full text of this publication is not available from this repository.

Book section

Scott, S.J. and Marchant, R.J. and Al-Fageeh, M.B. and Underhill, M.F. and Smales, C.M. (2006) The molecular response(s) during cellular adaptation to, and recovery from, sub-physiological temperatures. In: Al-Rubeai, M. and Fussenegger, M., eds. Systems Biology. Cell Engineering (5). Springer, Netherlands, pp. 185-212. ISBN 978-1-4020-5251-4. The full text of this publication is not available from this repository.

Alete, D.E. and Racher, R.J. and Birch, J.R. and James, D.C. and Smales, C.M. (2005) The functional competence of animal cells: Analysis of the secretory pathway. In: Godia, F. and Fussenegger, M., eds. Animal Cell Technology Meets Genomics. Springer, Dordrecht, pp. 71-74. ISBN 1-4020-2791-5 . The full text of this publication is not available from this repository.

Edited book

Smales, C.M. and James, D.C., eds. (2005) Characterization of therapeutic proteins by membrane and in-gel tryptic digestion. Methods in Molecular Biology, 308 . Humana Press, 482 pp. ISBN 9781588293909. The full text of this publication is not available from this repository.

This list was generated on Mon May 20 03:20:00 2013 BST.