The role of Plo1 kinase in mitotic commitment and septation in Schizosaccharomyces pombe

Tanaka, Kayoko and Petersen, Janni and MacIver, Fiona and Mulvihill, Daniel P. and Glover, David M. and Hagan, Iain M. (2001) The role of Plo1 kinase in mitotic commitment and septation in Schizosaccharomyces pombe. Embo Journal, 20 (6). pp. 1259-70. ISSN 0261-4189 . (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1093/emboj/20.6.1259

Abstract

Plo1-associated casein kinase activity peaked during mitosis before septation. Phosphatase treatment abolished this activity. Mitotic Plo1 activation had a requirement for prior activation of M-phase promoting factor (MPF), suggesting that Plo1 does not act as a mitotic trigger kinase to initiate MPF activation during mitotic commitment. A link between Plo1 and the septum initiating network (SIN) has been suggested by the inability of plo1 Delta cells to septate and the prolific septation following plo1(+) overexpression. Interphase activation of Spg1, the G protein that modulates SIN activity, induced septation but did not stimulate Plo1-associated kinase activity. Conversely, SIN inactivation did not affect the mitotic stimulation of Plo1-associated kinase activity. plo1.ts4 cells formed a misshapen actin ring, but rarely septated at 36 degrees C. Forced activation of Spg1 enabled plo1.ts4 mutant cells, but not cells with defects in the SIN component Sid2, to convert the actin ring to a septum. The ability of plo1(+) overexpression to induce septation was severely compromised by SIN inactivation. We propose that Plo1 acts before the SIN to control septation.

Item Type: Article
Additional information: 0261-4189 (Print) Journal Article Research Support, Non-U.S. Gov't
Uncontrolled keywords: CDC2 Protein Kinase/metabolism Casein Kinases Cell Cycle Proteins/metabolism *Drosophila Proteins Enzyme Activation Fungal Proteins/metabolism Maturation-Promoting Factor/metabolism *Mitosis Phenotype Protein Kinases/*metabolism Protein-Serine-Threonine Kinases/*metabolism Schizosaccharomyces/*cytology ras-GRF1/metabolism
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: Dan Mulvihill
Date Deposited: 27 Oct 2008 12:14
Last Modified: 16 Apr 2014 14:13
Resource URI: http://kar.kent.ac.uk/id/eprint/8912 (The current URI for this page, for reference purposes)
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