Bennett, Pauline M. and Baines, A.J. and Lecomte, Marie-Christine and Maggs, Alison M. and Pinder, Jennifer C. (2004) Not just a plasma-membrane protein - in cardiac muscle cell alpha-II spectrin also shows a close association with myofibrils. Journal of muscle research and cell motility, 25 (2). pp. 119-126. ISSN 0142-4319.
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Spectrin and its associated proteins are essential for the integrity of muscle cells and there is increasing evidence for their involvement in signalling pathways as well as having a structural function in mediating stress. Spectrin is a multigene family and it is essential to determine which isoforms are present and their location in the cell. In heart muscle, we have found that one spectrin isoform, alphaII-spectrin, is strongly represented and, using immunofluorescence, we show that it lies within the contractile fibres near the Z-disc as well as on the cardiomyocyte plasma membrane. Electron microscopy of immunogold-labelled cryosections reveals statistically significant clustering of gold particles near the Z-disc, within and close to the edge of myofibrils. betaII-spectrin and ankyrin-R and G are both known to occupy this region. We suggest that alphaIIbetaII spectrin tetramers with ankyrin organise and/or stabilise cardiac muscle cell membrane components relative to the contractile apparatus.
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences > Cell & Developmental Biology Group|
|Depositing User:||Anthony Baines|
|Date Deposited:||17 Sep 2008 15:15|
|Last Modified:||17 Apr 2012 14:16|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/8633 (The current URI for this page, for reference purposes)|
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