Molecular tools for speciation and epidemiological studies of Acanthamoeba

Khan, N.A. and Paget, T.A. (2002) Molecular tools for speciation and epidemiological studies of Acanthamoeba. Current Microbiology, 44 (6). pp. 444-449. ISSN 0343-8651. (The full text of this publication is not available from this repository)

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Abstract

Current diagnostic methods for Acanthamoeba identification rely heavily on light microscopic techniques that do not provide sufficient information about the identification of Acanthamoeba at the species level, thus delaying accurate identification of the infective agent. Here we report the use of polymerase chain reaction (PCR)-based restriction enzyme analyses to detect and speciate Acanthamoeba from both clinical and environmental sources by comparing their restriction endonuclease patterns. Significant diversity was observed between and within morphologically defined Acanthamoeba species. The usefulness of PCR-based assays and other available diagnostic methods is discussed.

Item Type: Article
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > Medway School of Pharmacy
Depositing User: Tim Paget
Date Deposited: 24 Sep 2008 12:04
Last Modified: 14 Jan 2010 14:29
Resource URI: http://kar.kent.ac.uk/id/eprint/8106 (The current URI for this page, for reference purposes)
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