Discrimination and taxonomy of geographically diverse strains of nitrile-metabolizing actinomycetes using chemometric and molecular sequencing techniques

Brandao, P.F. and Clapp, J.P. and Bull, A.T. (2002) Discrimination and taxonomy of geographically diverse strains of nitrile-metabolizing actinomycetes using chemometric and molecular sequencing techniques. Environmental Microbiology, 4 (5). pp. 262-276. ISSN 1462-2912 . (The full text of this publication is not available from this repository)

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Abstract

Mycolic acid-containing actinomycetes capable of metabolizing nitriles were recovered from deep-sea sediments and terrestrial soils by enrichment culture on acetonitrile, benzonitrile, succinonitrile or bromoxynil. A total of 43 nitrile-degrading strains were isolated and, together with previously recovered nitrile-degrading rhodococci, were identified by a polyphasic taxonomic approach, which included mycolic acid profiles, pyrolysis mass spectrometry (PyMS), genomic fingerprinting based on sequence variability of the 16S ribosomal RNA gene using polymerase chain reaction-restriction fragment length polymorphism-single-strand conformational polymorphism, and 16S rRNA gene sequence comparison. Isolates phylogenetically related to Rhodococcus erythropolis dominated the culturable microorganisms from most marine and terrestrial samples. These isolates clustered together in a major pyrogroup that showed high congruence with PRS profiles of the 16S rRNA gene. Such high congruence also was obtained for other recovered isolates that were assigned to species of Rhodococcus and Gordonia. Sequencing data validated the results obtained by PRS analysis and enabled phylogenetic relationships to be established. Some of the recovered bacteria probably represent novel microbial species. The fact that nitrile-metabolizing microorganisms were recovered from a wide range of habitat types suggests that nitrile transforming enzymatic activity is geographically widely distributed in nature.

Item Type: Article
Additional information: 1462-2912 (Print) Comparative Study Journal Article Research Support, Non-U.S. Gov't
Uncontrolled keywords: Actinomycetales/*classification/genetics/metabolism Base Sequence DNA Fingerprinting DNA, Bacterial/chemistry/genetics Geologic Sediments/*microbiology Mass Spectrometry Molecular Sequence Data Nitriles/*metabolism Pacific Ocean Phylogeny Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Polymorphism, Single-Stranded Conformational RNA, Ribosomal, 16S/chemistry/genetics Sequence Analysis, DNA Variation (Genetics)
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences > Biomedical Research Group
Depositing User: Sue Davies
Date Deposited: 14 Sep 2008 15:52
Last Modified: 14 Jan 2010 14:26
Resource URI: http://kar.kent.ac.uk/id/eprint/7353 (The current URI for this page, for reference purposes)
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