Nitrile hydrolysing activities of deep-sea and terrestrial mycolate actinomycetes

Brandao, P.F. and Bull, A.T. (2003) Nitrile hydrolysing activities of deep-sea and terrestrial mycolate actinomycetes. Antonie Van Leeuwenhoek, 84 (2). pp. 89-98. ISSN 0003-6072. (The full text of this publication is not available from this repository)

The full text of this publication is not available from this repository. (Contact us about this Publication)
Official URL
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=...

Abstract

Nitrile metabolising actinomycetes previously recovered from deep-sea sediments and terrestrial soils were investigated for their nitrile transforming properties. Metabolic profiling and activity assays confirmed that all strains catalysed the hydrolysis of nitriles by a nitrile hydratase/amidase system. Acetonitrile and benzonitrile, when used as growth substrates for enzyme induction experiments, had a significant influence on the biotransformation activities towards various nitriles and amides. The specific activities of selected deep-sea and terrestrial acetonitrile-grown bacteria against a suite of nitriles and amides were higher than those of the only other reported marine nitrile-hydrolysing R. erythropolis, isolated from a shallow sediment. The increase of nitrile chain length appeared to have negative influence on the nitrile hydratase activity of acetonitrile-grown bacteria, but the same was not true for benzonitrile-grown bacteria. The nitrile hydratases and amidases were constitutive in 10 of the 16 deep-sea and terrestrial actinomycetes studied, and one strain showed an inducible hydratase and a constitutive amidase. Most of the deep-sea strains had constitutive activities and showed some of the highest activities and broadest substrate specificities of organisms included in this study.

Item Type: Article
Additional information: 0003-6072 (Print) Journal Article Research Support, Non-U.S. Gov't
Uncontrolled keywords: Acetamides/metabolism Acetonitriles/metabolism Actinobacteria/growth & development/isolation & purification/*metabolism Amidohydrolases/metabolism Biotransformation Geologic Sediments/microbiology Hydro-Lyases/metabolism Hydrolysis Kinetics Nitriles/*metabolism Rhodococcus/growth & development/isolation & purification/metabolism Seawater/microbiology Soil Microbiology Substrate Specificity
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences > Biomedical Research Group
Depositing User: Sue Davies
Date Deposited: 09 Sep 2008 08:57
Last Modified: 14 Jan 2010 14:26
Resource URI: http://kar.kent.ac.uk/id/eprint/7351 (The current URI for this page, for reference purposes)
  • Depositors only (login required):