Brandao, P.F. and Bull, A.T. (2003) Nitrile hydrolysing activities of deep-sea and terrestrial mycolate actinomycetes. Antonie Van Leeuwenhoek, 84 (2). pp. 89-98. ISSN 0003-6072.
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Nitrile metabolising actinomycetes previously recovered from deep-sea sediments and terrestrial soils were investigated for their nitrile transforming properties. Metabolic profiling and activity assays confirmed that all strains catalysed the hydrolysis of nitriles by a nitrile hydratase/amidase system. Acetonitrile and benzonitrile, when used as growth substrates for enzyme induction experiments, had a significant influence on the biotransformation activities towards various nitriles and amides. The specific activities of selected deep-sea and terrestrial acetonitrile-grown bacteria against a suite of nitriles and amides were higher than those of the only other reported marine nitrile-hydrolysing R. erythropolis, isolated from a shallow sediment. The increase of nitrile chain length appeared to have negative influence on the nitrile hydratase activity of acetonitrile-grown bacteria, but the same was not true for benzonitrile-grown bacteria. The nitrile hydratases and amidases were constitutive in 10 of the 16 deep-sea and terrestrial actinomycetes studied, and one strain showed an inducible hydratase and a constitutive amidase. Most of the deep-sea strains had constitutive activities and showed some of the highest activities and broadest substrate specificities of organisms included in this study.
|Additional information:||0003-6072 (Print) Journal Article Research Support, Non-U.S. Gov't|
|Uncontrolled keywords:||Acetamides/metabolism Acetonitriles/metabolism Actinobacteria/growth & development/isolation & purification/*metabolism Amidohydrolases/metabolism Biotransformation Geologic Sediments/microbiology Hydro-Lyases/metabolism Hydrolysis Kinetics Nitriles/*metabolism Rhodococcus/growth & development/isolation & purification/metabolism Seawater/microbiology Soil Microbiology Substrate Specificity|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences > Biomedical Research Group|
|Depositing User:||Sue Davies|
|Date Deposited:||09 Sep 2008 08:57|
|Last Modified:||14 Jan 2010 14:26|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/7351 (The current URI for this page, for reference purposes)|
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