Maldonado, L.A. and Stach, J.E.M. and Pathom-aree, W. and Ward, A.C. and Bull, A.T. and Goodfellow, M. (2005) Diversity of cultivable actinobacteria in geographically widespread marine sediments. Antonie Van Leeuwenhoek International Journal Of General And Molecular Microbiology, 87 (1). pp. 11-18. ISSN 0003-6072 .
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Reports describing actinobacteria isolated from marine environments have been dominated by Micromonospora, Rhodococcus and Streptomyces species. Recent culture-independent studies have shown that marine environments contain a high diversity of actinobacterial species that are rarely, if at all, recovered by cultivation-based methods. In this study, it is shown that cultivation-independent methods can be used to guide the application of selective isolation methods. The detection of marine-derived actinobacterial species that have previously only been reported from terrestrial habitats is highlighted. This study provides good evidence that the previously described low diversity of actinobacterial species isolated from marine environments does not reflect an actual low species diversity, and that the use of informed selective isolation procedures can aid in the isolation of members of novel taxa.
|Additional information:||Article 900RC English Times Cited:21 Cited References Count:47|
|Uncontrolled keywords:||16S rRNA gene Actinobacteria marine environment PCR selective isolation sequencing SELECTIVE ISOLATION OCEAN SEDIMENTS COMMUNITIES PRIMERS SOIL IDENTIFICATION SUBSURFACE STRATEGIES RIFAMPICIN NOCARDIA|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences > Biomedical Research Group|
|Depositing User:||Sue Davies|
|Date Deposited:||10 Sep 2008 23:02|
|Last Modified:||14 Jan 2010 14:26|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/7339 (The current URI for this page, for reference purposes)|
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