Durr, R. and Vielhauer, O. and Burton, S.G. and Cowan, D.A. and Punal, A. and Brandao, P.F.B. and Bull, A.T. and Syldatk, C. (2006) Distribution of hydantoinase activity in bacterial isolates from geographically distinct environmental sources. Journal of Molucular Catalysis B-Enzymatic, 39 (1-4). pp. 160-165. ISSN 1381-1177.
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Hydantoin cleaving bacterial isolates were recovered from terrestrial soil samples originating from different geographic sources (Antarctica, South Africa and China) using culture-based screening methods (selective agar plates and shake flask cultures supplemented with hydantoins). Thirty-two bacterial isolates possessing the capability to transform the model substrates benzylhydantoin and dihydrouracil to the corresponding N-carbamoyl-amino acids were successfully cultured. Amplification and sequencing of the 16S rDNA revealed that the isolates belonged to the genera Arthrobacter, Burkholderia, Bacillus, Delftia, Enterobacter, Flavobacterium, Ochrobactrum, Pseudomonas and Stenotrophomonas, with one isolate assigned to the family Microbacteriacae. We have shown that microorganisms with hydantoinase activity are: (i) distributed in various geographically distinct environmental habitats, (ii) distributed worldwide and (iii) found in certain bacterial genera. Furthermore, we have demonstrated the presence of hydantoinase activity in genera in which hydantoinase activity has not previously been reported.
|Additional information:||Special Issue: Sp. Iss. SI Article 037VF English Times Cited:0 Cited References Count:47|
|Uncontrolled keywords:||hydantoinase screening extreme environment|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences > Biomedical Research Group|
|Depositing User:||Sue Davies|
|Date Deposited:||05 Sep 2008 19:44|
|Last Modified:||14 Jan 2010 14:26|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/7337 (The current URI for this page, for reference purposes)|
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