Combined bioaugmentation and biostimulation to cleanup soil contaminated with high concentrations of atrazine

Silva, Elisabete and Fialho, Arsenio M. and Sa-Correia, Isabel and Burns, Richard G. and Shaw, Liz J. (2004) Combined bioaugmentation and biostimulation to cleanup soil contaminated with high concentrations of atrazine. Environmental Science and Technology, 38 (2). pp. 632-637. ISSN 0013-936X. (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1021/es0300822

Abstract

We developed a joint bioaugmentation and biostimulation approach for the clean up of soil contaminated with high (168.7 and 337.4 mug g(-1)) concentrations of the herbicide atrazine (2-chloro-4-(ethylamino)-6-isopropylamino-s-triazine). Pseudomonas sp. strain ADP (P. ADP) was used for bioaugmentation (-10(7) cells g(-1) soil), and citrate (concentration range 5.8-40 mg g(-1) soil) and succinate (6.2-30.8 mg g(-1)) were used for biostimulation. The study soil had indigenous potential for atrazine mineralization (54.4 +/- 2% of 168.7 mug g(-1) mineralized after 67 day), but rapid mineralization only took place after a prolonged acclimation phase (similar to28 days). Inoculation with P. ADP alone resulted in a limited improvement in mineralization (e.g., 30.6 +/- 1% mineralization of 168.7 mug g(-1) of atrazine in inoculated soil cf. <0.5% in noninoculated in 7 days). Quantification of surviving numbers of P. ADP revealed a 10-fold decline from initial levels. However, bioaugmentation together with citrate or succinate biostimulation markedly increased P. ADP cell survival and atrazine mineralization (e.g., addition of 11.6 mg g(-1) of citrate increased mineralization of 337.4 mug g(-1) of atrazine from <2 to 79.9 +/- 1% in 13 days). A critical parameter in determining the extent of atrazine mineralization by P. ADP was C-s:N-atz (soluble carbon to atrazine nitrogen ratio): C-s:N-atz > 40 was required for maximal atrazine mineralization. We suggest our observations may be used as a framework for rational bioremediation of field soils contaminated with atrazine.

Item Type: Article
Additional information: Article 763XB English Times Cited:12 Cited References Count:56
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: Sue Davies
Date Deposited: 10 Sep 2008 14:35
Last Modified: 16 Apr 2014 14:25
Resource URI: http://kar.kent.ac.uk/id/eprint/6743 (The current URI for this page, for reference purposes)
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