A soil-based microbial biofilm exposed to 2,4-D: bacterial community development and establishment of conjugative plasmid pJP4

Aspray, T.J. and Hansen, S.K. and Burns, R.G. (2005) A soil-based microbial biofilm exposed to 2,4-D: bacterial community development and establishment of conjugative plasmid pJP4. FEMS Microbiol Ecol, 54 (2). pp. 317-327. ISSN 0168-6496 . (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1016/j.femsec.2005.04.007

Abstract

A soil suspension was used as a source to initiate the development of microbial communities in flow cells irrigated with 2,4-dichlorophenoxyacetic acid (2,4-D) (25 microg ml(-1)). Culturable bacterial members of the community were identified by 16S rRNA gene sequencing and found to be members of the genera Pseudomonas, Burkholderia, Collimonas and Rhodococcus. A 2,4-D degrading donor strain, Pseudomonas putida SM1443 (pJP4:gfp), was inoculated into flow cell chambers containing 2-day old biofilm communities. Transfer of pJP4:gfp from the donor to the bacterial community was detectable as GFP fluorescing cells and images were captured using confocal scanning laser microscopy (GFP fluorescence was repressed in the donor due to the presence of a chromosomally located lacI(q) repressor gene). Approximately 5-10 transconjugant microcolonies, 20-40 microm in diameter, could be seen to develop in each chamber. A 2,4-D degrading transconjugant strain was isolated from the flow cell system belonging to the genus Burkholderia.

Item Type: Article
Additional information: 0168-6496 (Print) Journal Article
Uncontrolled keywords: 2,4-Dichlorophenoxyacetic Acid/*metabolism Bacteria/*genetics *Biofilms *Conjugation, Genetic *Plasmids Polymerase Chain Reaction Polymorphism, Single-Stranded Conformational *Soil Microbiology
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: Sue Davies
Date Deposited: 05 Sep 2008 12:15
Last Modified: 14 Jan 2010 14:25
Resource URI: http://kar.kent.ac.uk/id/eprint/6740 (The current URI for this page, for reference purposes)
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