Baker, K.N. and Rendall, M.H. and Patel, A. and Boyd, P. and Hoare, M. and Freedman, R.B. and James, D.C. (2002) Rapid monitoring of recombinant protein products: a comparison of current technologies. Trends Biotechnol, 20 (4). pp. 149-156. ISSN 0167-7799.
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Abstract
Specific measurement of recombinant protein titer in a complex environment during industrial bioprocessing has traditionally relied on labor-intensive and time-consuming immunoassays. In recent years, however, developments in analytical technology have resulted in improved methods for protein product monitoring during bioprocessing. The choice of product-monitoring technology for a particular bioprocess will depend on a variety of assay factors and instrument-specific factors. In this article, we have compiled an overview of the advantages and disadvantages of the most commonly used technologies used: electrochemiluminescence, optical biosensors, rapid chromatography and nephelometry. The advantages of each technology for measuring both small and large recombinant therapeutic proteins are compared with a conventional enzyme-linked immunosorbent assay (ELISA) technique.
| Item Type: | Article |
|---|---|
| Additional information: | 0167-7799 (Print) Comparative Study Journal Article |
| Uncontrolled keywords: | Biosensing Techniques/economics/methods/trends Chemiluminescent Measurements Electrochemistry/economics/instrumentation/trends Enzyme-Linked Immunosorbent Assay/economics/methods/trends Humans *Molecular Probe Techniques Nephelometry and Turbidimetry/economics/methods/trends Optics/instrumentation Recombinant Proteins/*analysis |
| Subjects: | Q Science |
| Divisions: | Faculties > Science Technology and Medical Studies > School of Biosciences > Protein Science Group |
| Depositing User: | Sue Davies |
| Date Deposited: | 09 Sep 2008 18:10 |
| Last Modified: | 14 Jan 2010 14:24 |
| Resource URI: | http://kar.kent.ac.uk/id/eprint/6496 (The current URI for this page, for reference purposes) |
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