Baker, K.N. and Rendall, M.H. and Patel, A. and Boyd, P. and Hoare, M. and Freedman, R.B. and James, D.C. (2002) Rapid monitoring of recombinant protein products: a comparison of current technologies. Trends Biotechnol, 20 (4). pp. 149-156. ISSN 0167-7799.
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Specific measurement of recombinant protein titer in a complex environment during industrial bioprocessing has traditionally relied on labor-intensive and time-consuming immunoassays. In recent years, however, developments in analytical technology have resulted in improved methods for protein product monitoring during bioprocessing. The choice of product-monitoring technology for a particular bioprocess will depend on a variety of assay factors and instrument-specific factors. In this article, we have compiled an overview of the advantages and disadvantages of the most commonly used technologies used: electrochemiluminescence, optical biosensors, rapid chromatography and nephelometry. The advantages of each technology for measuring both small and large recombinant therapeutic proteins are compared with a conventional enzyme-linked immunosorbent assay (ELISA) technique.
|Additional information:||0167-7799 (Print) Comparative Study Journal Article|
|Uncontrolled keywords:||Biosensing Techniques/economics/methods/trends Chemiluminescent Measurements Electrochemistry/economics/instrumentation/trends Enzyme-Linked Immunosorbent Assay/economics/methods/trends Humans *Molecular Probe Techniques Nephelometry and Turbidimetry/economics/methods/trends Optics/instrumentation Recombinant Proteins/*analysis|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences > Protein Science Group|
|Depositing User:||Sue Davies|
|Date Deposited:||09 Sep 2008 18:10|
|Last Modified:||14 Jan 2010 14:24|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/6496 (The current URI for this page, for reference purposes)|
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