Al-Fageeh, M.B. and Marchant, R.J. and Carden, M.J. and Smales, C.M. (2006) The cold-shock response in cultured mammalian cells: Harnessing the response for the improvement of recombinant protein production. Biotechnology and Bioengineering, 93 (5). pp. 829-835. ISSN 0006-3592.
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There are a growing number of reports on the sub-physiological temperature culturing (< 37 degrees C) of mammalian cells for increased recombinant protein yield, although the effect is variable between cell lines, expression systems, and the product of interest. What is becoming clear is that exposing mammalian cells to sub-physiological temperatures invokes a coordinated cellular response involving modulation of the cell cycle, metabolism, transcription, translation, and the cell cytoskeleton. Opportunities currently exist for further enhancement of the cold-shock effect on recombinant protein production in mammalian cells through advancements in our understanding of the mechanisms involved in the cold-shock response.
|Uncontrolled keywords:||cold-shock response; recombinant protein synthesis; mammalian cells; specific production rate; volumetric production|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||Mark Smales|
|Date Deposited:||03 Sep 2008 13:37|
|Last Modified:||14 Jan 2010 14:23|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/6229 (The current URI for this page, for reference purposes)|
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