Evaluation of individual protein errors in silver-stained two-dimensional gels

Smales, C.M. and Birch, J.R. and Racher, A.J. and Marshall, C.T. and James, D.C. (2003) Evaluation of individual protein errors in silver-stained two-dimensional gels. Biochemical and Biophysical Research Communications, 306 (4). pp. 1050-1055. ISSN 0006-291X. (The full text of this publication is not available from this repository)

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Abstract

The relationship between spot volume and variation for all protein spots observed on large format 2D gels when utilising silver stain technology and a model system based on mammalian NSO cell extracts is reported. By running multiple gels we have shown that the reproducibility of data generated in this way is dependent on individual protein spot volumes, which in turn are directly correlated with the coefficient of variation. The coefficients of variation across all observed protein spots were highest for low abundant proteins which are the primary contributors to process error, and lowest for more abundant proteins. Using the relationship between spot volume and coefficient of variation we show it is necessary to calculate variation for individual protein spot volumes. The inherent limitations of silver staining therefore mean that errors in individual protein spot volumes must be considered when assessing significant changes in protein spot volume and not global error. (C) 2003 Elsevier Science (USA). All rights reserved.

Item Type: Article
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: Mark Smales
Date Deposited: 09 Sep 2008 04:54
Last Modified: 14 Jan 2010 14:23
Resource URI: http://kar.kent.ac.uk/id/eprint/6213 (The current URI for this page, for reference purposes)
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