Acid-polyacrylamide gel electrophoresis of lysozyme-estrone glucuronide conjugates

Smales, C. Mark and Elgar, David F. and Moore, Christopher H. and Blackwell, Leonard F. (1998) Acid-polyacrylamide gel electrophoresis of lysozyme-estrone glucuronide conjugates. Bioconjugate Chemistry, 9 (6). pp. 838-841. ISSN 1043-1802. (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1021/bc9701914

Abstract

Acid-polyacrylamide gel electrophoresis (acid-PACE) was used for analysis of lysozyme-estrone glucuronide conjugates. The resolution of the system allowed the identification of individual conjugate families which differed only in the position of acylation or in the number of estrone glucuronide units. Acid-PAGE was a good alternative to denaturing cation-exchange chromatography for the analysis, separation, and small-scale purification of lysozyme-estrone glucuronide conjugates. It revealed the true order of the relative degree of positive charge on the lysozyme-estrone glucuronide conjugates.

Item Type: Article
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: Mark Smales
Date Deposited: 25 Jun 2009 08:34
Last Modified: 09 Jun 2014 08:38
Resource URI: http://kar.kent.ac.uk/id/eprint/6203 (The current URI for this page, for reference purposes)
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