Lotz, H. and Sohn, K. and Brunner, H. and Muhlschlegel, F.A. and Rupp, S. (2004) RBR1, a novel pH-regulated cell wall gene of Candida albicans, is repressed by RM101 and activated by NRG1. EUKARYOTIC CELL, 3 (3). pp. 776-784. ISSN 1535-9778.
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The transcription factor Rim101p of Candida albicans has been shown to play a major role in pH-dependent gene regulation. Rim101p is involved in cell wall biosynthesis, since it regulates PHR1 and PHR2, two almost functionally redundant cell wall glycosidases important for adaptation to either neutral or acidic habitats within the human host. To identify additional cell wall components regulated by Rim101p, we performed transcriptional profiling with a cell wall-specific DNA microarray. We showed that Rim101p contributes to the activation of known hypha-specific genes such as HWP1 and RBT1 but is also required for repression of the previously uncharacterized potential cell wall genes RBR1, RBR2, and RBR3. Further characterization of RBR1 revealed that it encodes a small glycosylphosphatidyl inositol protein that is expressed under acidic conditions predominantly at low temperature. Deletion of the gene resulted in a filamentation defect at low pH. Most interestingly, NRG1, a transcriptional repressor of hyphal growth in C. albicans, was required for RBR1 expression. The apparently activating effect of NRG1 observed in this study has not been described before. In addition, we showed that expression of NRG1 is not only temperature but also pH dependent.
|Additional information:||1535-9778 (Print) Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.|
|Uncontrolled keywords:||Blotting, Southern Candida albicans/*genetics/metabolism Cell Wall/*genetics/metabolism DNA Primers/genetics DNA-Binding Proteins/genetics/*metabolism Fungal Proteins/*genetics/metabolism Gene Expression Regulation, Fungal/genetics Hydrogen-Ion Concentration Hyphae/genetics Membrane Glycoproteins/*genetics Mutation/genetics Oligonucleotide Array Sequence Analysis Repressor Proteins/genetics/*metabolism Transcription Factors/genetics/metabolism|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences > Biomedical Research Group|
|Depositing User:||F.A. Muhlschlegel|
|Date Deposited:||09 Sep 2008 16:12|
|Last Modified:||14 Jan 2010 14:23|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/6195 (The current URI for this page, for reference purposes)|
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