Candida glabrata STE12 is required for wild-type levels of virulence and nitrogen starvation induced filamentation

Calcagno, Ana-Maria and Bignell, Elaine and Warn, Peter and Jones, Michael D. and Denning, David W. and Mühlschlegel, Fritz A. and Rogers, Thomas R. and Haynes, Ken (2003) Candida glabrata STE12 is required for wild-type levels of virulence and nitrogen starvation induced filamentation. Molecular Microbiology, 50 (4). pp. 1309-1318. ISSN 0950-382X. (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1046/j.1365-2958.2003.03755.x

Abstract

The highly conserved fungal Ste12 transcription factor family of proteins play critical roles in the regulation of many cellular processes including mating, cell wall biosynthesis, filamentation and invasive growth. They are also important mediators of fungal virulence. The Candida glabrata STE12 homologue was cloned. The encoded protein has a single DNA binding homeodomain but lacks both a C2H2 zinc finger DNA binding domain and an apparent Dig1/Dig2 regulatory motif. Candida glabrata STE12 can functionally complement the nitrogen starvation induced filamentation and mating defects of Saccharomyces cerevisiae ste12 mutants. We also show that C. glabrata STE12 is required for nitrogen starvation-induced filamentation as ste12 mutants rarely produce pseudohyphae on nitrogen depleted media. Finally we describe a novel murine model of C. glabrata systemic disease and use this to demonstrate that C. glabrata ste12 mutants, although still able to cause disease, are attenuated for virulence compared with STE12 reconstituted strains. Candida glabrata STE12 is therefore the first virulence factor encoding gene to be described in this increasingly important fungal pathogen.

Item Type: Article
Additional information: 0950-382X (Print) Journal Article Research Support, Non-U.S. Gov't
Uncontrolled keywords: Animals Candida glabrata/cytology/genetics/*metabolism/*pathogenicity Candidiasis/microbiology/pathology Fungal Proteins/classification/genetics/*metabolism Genetic Complementation Test Liver/microbiology/pathology Mice Nitrogen/*metabolism Phylogeny Saccharomyces cerevisiae/genetics/metabolism *Saccharomyces cerevisiae Proteins Transcription Factors/classification/genetics/*metabolism Virulence Factors/classification/*genetics/metabolism
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Faculties > Science Technology and Medical Studies > School of Biosciences > Biomedical Research Group
Depositing User: F.A.M. Muhlschlegel
Date Deposited: 09 Sep 2008 04:34
Last Modified: 30 Apr 2014 09:28
Resource URI: http://kar.kent.ac.uk/id/eprint/6192 (The current URI for this page, for reference purposes)
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