Calcagno, A.M. and Bignell, E. and Rogers, T.R. and Canedo, M. and Muhlschlegel, F.A. and Haynes, K. (2004) Candida glabrata Ste20 is involved in maintaining cell wall integrity and adaptation to hypertonic stress, and is required for wild-type levels of virulence. Yeast, 21 (7). pp. 557-568. ISSN 0749-503X.
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The conserved family of fungal Ste20 p21-activated serine-threonine protein kinases regulate several signalling cascades. In Saccharomyces cerevisiae Ste20 is involved in pheromone signalling, invasive growth, the hypertonic stress response, cell wall integrity and binds Cdc42, a Rho-like small GTP-binding protein required for polarized morphogenesis. We have cloned the STE20 homologue from the fungal pathogen Candida glabrata and have shown that it is present in a single copy in the genome. Translation of the nucleotide sequence predicts that C. glabrata Ste20 contains a highly conserved p21-activated serine-threonine protein kinase domain, a binding site for G-protein beta subunits and a regulatory Rho-binding domain that enables the kinase to interact with Cdc42 and/or Rho-like small GTPases. C. glabrata Ste20 has 53% identity and 58% predicted amino acid similarity to S. cerevisiae Ste20 and can complement both the nitrogen starvation-induced filamentation and mating defects of S. cerevisiae ste20 mutants. Analysis of ste20 null and disrupted strains suggest that in C. glabrata Ste20 is required for a fully functional hypertonic stress response and intact cell wall integrity pathway. C. glabrata Ste20 is not required for nitrogen starvation-induced filamentation. Survival analysis revealed that C. glabrata ste20 mutants, while still able to cause disease, are mildly attenuated for virulence compared to reconstituted STE20 cells.
|Additional information:||0749-503X (Print) Journal Article Research Support, Non-U.S. Gov't|
|Uncontrolled keywords:||Amino Acid Sequence Animals Base Sequence Candida glabrata/enzymology/genetics/pathogenicity/*physiology Candidiasis/*microbiology Cell Wall/enzymology/physiology Cloning, Molecular DNA, Fungal/chemistry/genetics Fungal Proteins/genetics/*physiology Genetic Complementation Test Mice Molecular Sequence Data Mutagenesis, Insertional Polymerase Chain Reaction Protein-Serine-Threonine Kinases/genetics/*physiology Recombinant Proteins *Saccharomyces cerevisiae Proteins Sequence Alignment Virulence|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||F.A. Muhlschlegel|
|Date Deposited:||09 Sep 2008 16:04|
|Last Modified:||14 Jan 2010 14:23|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/6190 (The current URI for this page, for reference purposes)|
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