Global changes in gene expression observed at the transition from growth to stationary phase in Listeria monocytogenes ScottA batch culture

Weeks, Mark E. and James, David C. and Robinson, Gary K. and Smales, C. Mark (2004) Global changes in gene expression observed at the transition from growth to stationary phase in Listeria monocytogenes ScottA batch culture. Proteomics, 4 (1). pp. 123-135. ISSN 1615-9853. (Access to this publication is restricted)

PDF
Restricted to Repository staff only
Contact us about this Publication Download (325kB)
[img]
Official URL
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=...

Abstract

Listeria monocytogenes is a food-borne Gram-positive bacterium that is responsible for a variety of infections (worldwide) annually. The organism is able to survive a variety of environmental conditions and stresses, however, the mechanisms by which L. monocytogenes adapts to environmental change are yet to be fully elucidated. An understanding of the mechanism(s) by which L. monocytogenes survives unfavourable environmental conditions will aid in developing new food processing methods to control the organism in foodstuffs. We have utilized a proteomic approach to investigate the response of L. monocytogenes batch cultures to the transition from exponential to stationary growth phase. Proteomic analysis showed that batch cultures of L. monocytogenes perceived stress and began preparations for stationary phase much earlier (approximately A(600) = 0.75, mid-exponential) than predicted by growth characteristics alone. Global analysis of the proteome revealed that the expression levels of more than 50% of all proteins observed changed significantly over a 7-9 h period during this transition phase. We have highlighted ten proteins in particular whose expression levels appear to be important in the early onset of the stationary phase. The significance of these findings in terms of functionality and the mechanistic picture are discussed.

Item Type: Article
Uncontrolled keywords: environmental adaptation; growth phase; Listeria monocytogenes; two-dimensional gel electrophoresis
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences > Protein Science Group
Depositing User: Sue Davies
Date Deposited: 19 Dec 2007 17:51
Last Modified: 23 May 2014 13:09
Resource URI: http://kar.kent.ac.uk/id/eprint/61 (The current URI for this page, for reference purposes)
  • Depositors only (login required):

Downloads

Downloads per month over past year