Differential localization of glutamate receptor subunits at the Drosophila neuromuscular junction

Marrus, S.B. and Portman, S.L. and Allen, M.J. and Moffat, K.G. and DiAntonio, A. (2004) Differential localization of glutamate receptor subunits at the Drosophila neuromuscular junction. JOURNAL OF NEUROSCIENCE, 24 (6). pp. 1406-1415. ISSN 0270-6474. (The full text of this publication is not available from this repository)

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Abstract

The subunit composition of postsynaptic neurotransmitter receptors is a key determinant of synaptic physiology. Two glutamate receptor subunits, Drosophila glutamate receptor IIA (DGluRIIA) and DGluRIIB, are expressed at the Drosophila neuromuscular junction and are redundant for viability, yet differ in their physiological properties. We now identify a third glutamate receptor subunit at the Drosophila neuromuscular junction, DGluRIII, which is essential for viability. DGluRIII is required for the synaptic localization of DGluRIIA and DGluRIIB and for synaptic transmission. Either DGluRIIA or DGluRIIB, but not both, is required for the synaptic localization of DGluRIII. DGluRIIA and DGluRIIB compete with each other for access to DGluRIII and subsequent localization to the synapse. These results are consistent with a model of a multimeric receptor in which DGluRIII is an essential component. At single postsynaptic cells that receive innervation from multiple motoneurons, DGluRIII is abundant at all synapses. However, DGluRIIA and DGluRIIB are differentially localized at the postsynaptic density opposite distinct motoneurons. Hence, innervating motoneurons may regulate the subunit composition of their receptor fields within a shared postsynaptic cell. The capacity of presynaptic inputs to shape the subunit composition of postsynaptic receptors could be an important mechanism for synapse-specific regulation of synaptic function and plasticity.

Item Type: Article
Additional information: eng Journal Article
Uncontrolled keywords: Amino Acid Sequence Animals Animals, Genetically Modified Drosophila/*physiology Drosophila Proteins/*biosynthesis/genetics Electrophysiology Genetic Techniques Larva/physiology Molecular Sequence Data Muscles/metabolism/physiology Mutagenesis Neuromuscular Junction/*metabolism Protein Subunits/biosynthesis/genetics Receptors, Glutamate/*biosynthesis/genetics Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Sequence Homology, Amino Acid Synaptic Transmission/genetics/physiology
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences > Cell & Developmental Biology Group
Depositing User: Marcus J Allen
Date Deposited: 09 Sep 2008 15:32
Last Modified: 14 Jan 2010 14:21
Resource URI: http://kar.kent.ac.uk/id/eprint/5875 (The current URI for this page, for reference purposes)
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