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The Most Prevalent Freeman-Sheldon Syndrome Mutations in the Embryonic Myosin Motor Share Functional Defects.

Walklate, Jonathan, Vera, Carlos, Bloemink, Marieke J., Geeves, Michael A., Leinwand, Leslie (2016) The Most Prevalent Freeman-Sheldon Syndrome Mutations in the Embryonic Myosin Motor Share Functional Defects. Journal of Biological Chemistry, 291 (19). pp. 10318-10331. ISSN 0021-9258. (doi:10.1074/jbc.M115.707489) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:55461)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL:
http://doi.org/10.1074/jbc.M115.707489

Abstract

The embryonic myosin isoform is expressed during fetal development and rapidly down-regulated after birth. Freeman-Sheldon syndrome (FSS) is a disease associated with missense mutations in the motor domain of this myosin. It is the most severe form of distal arthrogryposis, leading to overcontraction of the hands, feet, and orofacial muscles and other joints of the body. Availability of human embryonic muscle tissue has been a limiting factor in investigating the properties of this isoform and its mutations. Using a recombinant expression system, we have studied homogeneous samples of human motors for the WT and three of the most common FSS mutants: R672H, R672C, and T178I. Our data suggest that the WT embryonic myosin motor is similar in contractile speed to the slow type I/? cardiac based on the rate constant for ADP release and ADP affinity for actin-myosin. All three FSS mutations show dramatic changes in kinetic properties, most notably the slowing of the apparent ATP hydrolysis step (reduced 5–9-fold), leading to a longer lived detached state and a slowed Vmax of the ATPase (2–35-fold), indicating a slower cycling time. These mutations therefore seriously disrupt myosin function.

Item Type: Article
DOI/Identification number: 10.1074/jbc.M115.707489
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Susan Davies
Date Deposited: 17 May 2016 11:16 UTC
Last Modified: 17 Aug 2022 12:20 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/55461 (The current URI for this page, for reference purposes)

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