Cox, B. and Ness, F. and Tuite, M.F. (2003) Analysis of the generation and segregation of propagons: entities that propagate the [PSI+] prion in yeast. Genetics, 165 (1). pp. 23-33. ISSN 0016-6731.
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The propagation of the prion form of the yeast Sup35p protein, the so-called [PSI(+)] determinant, involves the generation and partition of a small number of particulate determinants that we propose calling "propagons." The numbers of propagons in [PSI(+)] cells can be inferred from the kinetics of elimination of [PSI(+)] during growth in the presence of a low concentration of guanidine hydrochloride (GdnHCl). Using this and an alternative method of counting the numbers of propagons, we demonstrate considerable clonal variation in the apparent numbers of propagons between different [PSI(+)] yeast strains, between different cultures of the same [PSI(+)] yeast strain, and between different cells of the same [PSI(+)] culture. We provide further evidence that propagon generation is blocked by growth in GdnHCl and that it is largely confined to the S phase of the cell cycle. In addition, we show that at low propagon number there is a bias toward retention of propagons in mother cells and that production of new propagons is very rapid when cells with depleted numbers of propagons are rescued into normal growth medium. The implications of our findings with respect to yeast prion propagation mechanisms are discussed.
|Additional information:||0016-6731 (Print) Journal Article Research Support, Non-U.S. Gov't|
|Uncontrolled keywords:||Chromosome Segregation Gene Dosage Guanidine/metabolism Prions/*genetics/metabolism Saccharomyces cerevisiae/*genetics/metabolism Saccharomyces cerevisiae Proteins/*genetics/metabolism|
|Subjects:||Q Science > QR Microbiology|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences > Protein Science Group|
|Depositing User:||Mick Tuite|
|Date Deposited:||09 Sep 2008 04:00|
|Last Modified:||14 Jan 2010 14:20|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/5471 (The current URI for this page, for reference purposes)|
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