O'Sullivan, J.M. and Mihr, M.J. and Santos, M.A. and Tuite, M.F. (2001) Seryl-tRNA Synthetase is not responsible for the Evolution of CUG Codon Reassignment in Candida Albicans. Yeast, 18 (4). pp. 313-322. ISSN 0749-503X.
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A number of Candida species translate the standard leucine-CUG codon as serine using a novel ser-tRNA(CAG). This tRNA, which has an unusual anticodon stem-loop structure, has been implicated in the evolution of this codon reassignment. However, such a sense codon reassignment might also require a change in the specificity of the cognate aminoacyl tRNA-synthetase, in this case the ser-tRNA synthetase. Here we describe the cloning and sequence analysis of the C. albicans seryl aminoacyl-tRNA synthetase (CaSerRS) gene (CaSES1). The predicted CaSerRS sequence shows a significant level of amino acid identity to SerRs from other organisms and fully complements a S. cerevisiae SerRS null strain without any apparent defect in growth rate. This suggests that the SerRS recognizes and charges S. cerevisiae ser-tRNAs with similar efficiency to that of the S. cerevisiae SerRS. Using an antibody raised against CaSerRS, we also demonstrate the presence of SerRS in a range of Candida spp. showing CUG codon reassignment. We conclude that the key element in CUG reassigment in Candida spp. is the tRNA that decodes the CUG codon rather than a SerRS structural change. The nucleotide sequence of the CaSES1 gene has been deposited at GenBank under Accession No. AF290915.
|Additional information:||0749-503X (Print) Journal Article Research Support, Non-U.S. Gov't|
|Uncontrolled keywords:||Amino Acid Sequence Candida albicans/*genetics Cloning, Molecular Evolution, Molecular Gene Expression *Genetic Code Genetic Complementation Test Molecular Sequence Data Protein Biosynthesis/*genetics RNA, Transfer/genetics Sequence Analysis, DNA Sequence Homology, Amino Acid Serine/*genetics Serine-tRNA Ligase/*genetics/metabolism|
|Subjects:||Q Science > QR Microbiology|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences > Protein Science Group|
|Depositing User:||Mick Tuite|
|Date Deposited:||28 Oct 2008 18:47|
|Last Modified:||14 Jan 2010 14:20|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/5466 (The current URI for this page, for reference purposes)|
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