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Seryl-tRNA Synthetase is not responsible for the Evolution of CUG Codon Reassignment in Candida Albicans

O'Sullivan, Justin M., Mihr, Marian J., Santos, Manuel, Tuite, Mick F. (2001) Seryl-tRNA Synthetase is not responsible for the Evolution of CUG Codon Reassignment in Candida Albicans. Yeast, 18 (4). pp. 313-322. ISSN 0749-503X. (doi:10.1002/1097-0061(20010315)18:4<313::AID-YEA673>3.0.CO;2-7) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:5466)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL:
http://dx.doi.org/10.1002/1097-0061(20010315)18:4<...

Abstract

A number of Candida species translate the standard leucine-CUG codon as serine using a novel ser-tRNA(CAG). This tRNA, which has an unusual anticodon stem-loop structure, has been implicated in the evolution of this codon reassignment. However, such a sense codon reassignment might also require a change in the specificity of the cognate aminoacyl tRNA-synthetase, in this case the ser-tRNA synthetase. Here we describe the cloning and sequence analysis of the C. albicans seryl aminoacyl-tRNA synthetase (CaSerRS) gene (CaSES1). The predicted CaSerRS sequence shows a significant level of amino acid identity to SerRs from other organisms and fully complements a S. cerevisiae SerRS null strain without any apparent defect in growth rate. This suggests that the SerRS recognizes and charges S. cerevisiae ser-tRNAs with similar efficiency to that of the S. cerevisiae SerRS. Using an antibody raised against CaSerRS, we also demonstrate the presence of SerRS in a range of Candida spp. showing CUG codon reassignment. We conclude that the key element in CUG reassigment in Candida spp. is the tRNA that decodes the CUG codon rather than a SerRS structural change. The nucleotide sequence of the CaSES1 gene has been deposited at GenBank under Accession No. AF290915.

Item Type: Article
DOI/Identification number: 10.1002/1097-0061(20010315)18:4<313::AID-YEA673>3.0.CO;2-7
Additional information: 0749-503X (Print) Journal Article Research Support, Non-U.S. Gov't
Uncontrolled keywords: Amino Acid Sequence Candida albicans/*genetics Cloning, Molecular Evolution, Molecular Gene Expression *Genetic Code Genetic Complementation Test Molecular Sequence Data Protein Biosynthesis/*genetics RNA, Transfer/genetics Sequence Analysis, DNA Sequence Homology, Amino Acid Serine/*genetics Serine-tRNA Ligase/*genetics/metabolism
Subjects: Q Science > QR Microbiology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Michael Tuite
Date Deposited: 28 Oct 2008 18:47 UTC
Last Modified: 16 Nov 2021 09:43 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/5466 (The current URI for this page, for reference purposes)

University of Kent Author Information

Santos, Manuel.

Creator's ORCID:
CReDIT Contributor Roles:

Tuite, Mick F..

Creator's ORCID: https://orcid.org/0000-0002-5214-540X
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