Tuite, M.F. and Cox, B.S. (2006) The [PSI+] prion of yeast: a problem of inheritance. Methods, 39 (1). pp. 9-22. ISSN 1046-2023.
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| Official URL http://dx.doi.org/10.1016/j.ymeth.2006.04.001 |
Abstract
The [PSI(+)] prion of the yeast Saccharomyces cerevisiae was first identified by Brian Cox some 40 years ago as a non-Mendelian genetic element that modulated the efficiency of nonsense suppression. Following the suggestion by Reed Wickner in 1994 that such elements might be accounted for by invoking a prion-based model, it was subsequently established that the [PSI(+)] determinant was the prion form of the Sup35p protein. In this article, we review how a combination of classical genetic approaches and modern molecular and biochemical methods has provided conclusive evidence of the prion basis of the [PSI(+)] determinant. In so doing we have tried to provide a historical context, but also describe the results of more recent experiments aimed at elucidating the mechanism by which the [PSI(+)] (and other yeast prions) are efficiently propagated in dividing cells. While understanding of the [PSI(+)] prion and its mode of propagation has, and will continue to have, an impact on mammalian prion biology nevertheless the very existence of a protein-based mechanism that can have a beneficial impact on a cell's fitness provides equally sound justification to fully explore yeast prions.
| Item Type: | Article |
|---|---|
| Additional information: | 1046-2023 (Print) Journal Article Research Support, Non-U.S. Gov't |
| Uncontrolled keywords: | Amino Acid Sequence Cell Division/genetics/physiology Models, Biological Molecular Chaperones/metabolism Molecular Sequence Data Mutation/genetics Phenotype Prions/chemistry/*genetics/metabolism Saccharomyces cerevisiae/*genetics/growth & development/metabolism Saccharomyces cerevisiae Proteins/chemistry/*genetics/metabolism |
| Subjects: | Q Science > QR Microbiology |
| Divisions: | Faculties > Science Technology and Medical Studies > School of Biosciences > Protein Science Group |
| Depositing User: | Mick Tuite |
| Date Deposited: | 02 Sep 2008 23:06 |
| Last Modified: | 14 Jan 2010 14:20 |
| Resource URI: | http://kar.kent.ac.uk/id/eprint/5449 (The current URI for this page, for reference purposes) |
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