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Developments in the production of mucosal antibodies in plants.

Vasilev, Nikolay, Smales, Christopher Mark, Schillberg, Stefan, Fischer, Rainer, Schiermeyer, Andreas (2016) Developments in the production of mucosal antibodies in plants. Biotechnology Advances, 34 (2). pp. 77-87. ISSN 0734-9750. (doi:10.1016/j.biotechadv.2015.11.002) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:54273)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL:
http://doi.org/10.1016/j.biotechadv.2015.11.002

Abstract

Recombinant mucosal antibodies represent attractive target molecules for the development of next generation biopharmaceuticals for passive immunization against various infectious diseases and treatment of patients suffering from mucosal antibody deficiencies. As these polymeric antibodies require complex post-translational modifications and correct subunit assembly, they are considered as difficult-to-produce recombinant proteins. Beside the traditional, mammalian-based production platforms, plants are emerging as alternative expression hosts for this type of complex macromolecule. Plant cells are able to produce high-quality mucosal antibodies as shownby the successful expression of the secretory immunoglobulins A (IgA) andM(IgM) in various antibody formats in different plant species including tobacco and its close relative Nicotiana benthamiana, maize, tomato

and Arabidopsis thaliana. Importantly for biotherapeutic application, transgenic plants are capable of synthesizing functional IgA and IgM molecules with biological activity and safety profiles comparable with their native mammalian counterparts. This article reviews the structure and function of mucosal IgA and IgM antibodies and summarizes

the current knowledge of their production and processing in plant host systems. Specific emphasis is given to consideration of intracellular transport processes as these affect assembly of the mature immunoglobulins, their secretion rates, proteolysis/degradation and glycosylation patterns. Furthermore, this review provides an outline of glycoengineering efforts that have been undertaken so far to produce antibodies with homogenous human-like glycan decoration.Webelieve that the continued development of our understanding of the plant cellular machinery related to the heterologous expression of immunoglobulins will further improve the production levels, quality and control of post-translational modifications that are ‘human-like’ from plant systems and enhance the prospects for the regulatory approval of such molecules leading to the commercial exploitation of plant-derived mucosal antibodies.

Item Type: Article
DOI/Identification number: 10.1016/j.biotechadv.2015.11.002
Uncontrolled keywords: Biopharmaceuticals; Immunoglobulin A; Immunoglobulin M; Plant glycoengineering; Plant-made recombinant proteins; Secretory antibodies; Transgenic plants
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Mark Smales
Date Deposited: 24 Feb 2016 15:35 UTC
Last Modified: 17 Aug 2022 12:20 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/54273 (The current URI for this page, for reference purposes)

University of Kent Author Information

Smales, Christopher Mark.

Creator's ORCID: https://orcid.org/0000-0002-2762-4724
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