Wagstaff, J.L. and Sadovskaya, I. and Vinogradov, E. and Jabbouri, S. and Howard, M.J. (2008) Poly-N-acetylglucosamine and poly(glycerol phosphate) teichoic acid identification from staphylococcal biofilm extracts using excitation sculptured TOCSY NMR. Molecular Biosystems, 4 (2). pp. 170-174. ISSN 1742-206X.
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We report the successful application of selective excitation sculptured TOCSY NMR (SXS-TOCSY) to identify individual solution components from a heterogeneous system using selectively acquired 1 H NMR spin system patterns. SXS-TOCSY application is illustrated by detection of the simultaneous presence of poly-beta-(1,6)-N-acetylglucosamine (PNAG) and poly(glycerol phosphate) teichoic acid (TA) carbohydrate polymer components in crude biofilm extracts from Staphylococcus epidermidis without the need for further sample purification and component separation. Biofilms are implicated in the barriers for resistance of microbes toward antibiotics and immune responses, therefore efficient rapid detection and quantification of key components are important to assist in the design of a clinical infection response.
|Uncontrolled keywords:||coagulase-negative staphylococci structural elucidation intercellular adhesin epidermidis rp62a strain polysaccharide metabonomics components aureus|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences > Protein Science Group|
|Depositing User:||Mark Howard|
|Date Deposited:||13 Mar 2009 11:44|
|Last Modified:||21 May 2011 00:00|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/5355 (The current URI for this page, for reference purposes)|
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