Sheth, C.C. and Johnson, E. and Baker, M.E. and Haynes, K. and Muhlschlegel, F.A. (2005) Phenotypic identification of Candida albicans by growth on chocolate agar. Medical Mycology, 43 (8). pp. 735-738.
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In this study, we describe a simple method for the identification of Candida albicans in clinical samples. A total of 383 clinical isolates of Candida species were streaked onto chocolate agar and incubated for 48 h at 37 degrees C in the presence of an atmosphere of 6% CO2. All 208 of the C. albicans isolates tested, developed an easy to identify filamentous colony morphology. Of 175 other Candida species tested, 172 (98.3%) were distinguishable from C. albicans by their smooth colony morphology. Three isolates (1.7%) exhibited weak filamentation after prolonged incubation. Although not a routine medium in medical mycology a significant advantage of using chocolate agar lies in its use in clinical bacteriology laboratories for the isolation of fastidious bacteria. Implementation of the proposed method is applicable across a range of specimen types, thus allowing the direct identification of C. albicans in clinical samples. This simple method may allow a quicker entry into directed treatment.
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences > Biomedical Research Group|
|Depositing User:||Sue Davies|
|Date Deposited:||19 Dec 2007 17:49|
|Last Modified:||28 Mar 2012 10:48|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/50 (The current URI for this page, for reference purposes)|
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