Phenotypic identification of Candida albicans by growth on chocolate agar.

Sheth, C.C. and Johnson, E. and Baker, M.E. and Haynes, K. and Muhlschlegel, F.A. (2005) Phenotypic identification of Candida albicans by growth on chocolate agar. Medical Mycology, 43 (8). pp. 735-738. (Access to this publication is restricted)

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Abstract

In this study, we describe a simple method for the identification of Candida albicans in clinical samples. A total of 383 clinical isolates of Candida species were streaked onto chocolate agar and incubated for 48 h at 37 degrees C in the presence of an atmosphere of 6% CO2. All 208 of the C. albicans isolates tested, developed an easy to identify filamentous colony morphology. Of 175 other Candida species tested, 172 (98.3%) were distinguishable from C. albicans by their smooth colony morphology. Three isolates (1.7%) exhibited weak filamentation after prolonged incubation. Although not a routine medium in medical mycology a significant advantage of using chocolate agar lies in its use in clinical bacteriology laboratories for the isolation of fastidious bacteria. Implementation of the proposed method is applicable across a range of specimen types, thus allowing the direct identification of C. albicans in clinical samples. This simple method may allow a quicker entry into directed treatment.

Item Type: Article
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences > Biomedical Research Group
Depositing User: Sue Davies
Date Deposited: 19 Dec 2007 17:49
Last Modified: 28 Mar 2012 10:48
Resource URI: http://kar.kent.ac.uk/id/eprint/50 (The current URI for this page, for reference purposes)
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