Gibbs, B.F. and Wierecky, J. and Welker, P. and Henz, B.M. and Wolff, H.H. and Grabbe, J. (2001) Human Skin Mast Cells Release Preformed and Newly Generated TNF-α and IL-8 but not IL-4, IL-5 or IL-13 Following Stimulation with Anti-IgE and Other Secretagogues. Experimental Dermatology, 10 (5). pp. 312-320. ISSN 0906-6705.
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Several groups have previously reported that rodent or human leukemic mast cells produce inflammatory cytokines such as TNF-α and IL-8 as well as the pro-allergic cytokines IL-4, IL-5 and IL-13. Comparatively little is known, however, regarding the ability of normal human skin mast cells to secrete these factors following either IgE-dependent or IgE-independent modes of activation. We therefore investigated whether normal human skin mast cells produce these cytokines following stimulation by a variety of secretagogues. Enriched isolated skin mast cells released both TNF-α and IL-8 following activation with either anti-IgE, SCF, substance P, compound 48/80 or A23187. This release was dose- and time-dependent, with maximal levels being reached within 4 h of stimulation involving, in part, the secretion of preformed stores of both cytokines. In accordance with this, using lysates of highly purified (>90%) skin mast cells, we could demonstrate that both TNF-α and IL-8 mRNA and protein were present in both unstimulated as well as stimulated mast cells. In stark contrast to these results, no significant levels of either IL-4, IL-5 or IL-13 were detected, regardless of the secretagogue used or the period of stimulation. These results show that human skin mast cells are capable of rapidly secreting pro-inflammatory cytokines like TNF-α and IL-8 following IgE-dependent activation and stimulation by the neuropeptide substance P, SCF and the basic polypeptide analogue compound 48/80. In contrast to other types of human mast cells however, human skin mast cells were incapable of secreting IL-4, IL-5 or IL-13 in these settings.
Q Science > QR Microbiology > QR180 Immunology
|Divisions:||Faculties > Science Technology and Medical Studies > Medway School of Pharmacy|
|Depositing User:||Bernhard F- Gibbs|
|Date Deposited:||14 Sep 2008 09:31|
|Last Modified:||14 Jan 2010 14:17|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/4817 (The current URI for this page, for reference purposes)|
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