Skip to main content

Differential role for mitogen-activated protein kinases in IgE-dependent signalling in human peripheral blood basophils: in contrast to p38 MAPK, c-Jun N-terminal kinase is poorly expressed and does not appear to control mediator release.

Gibbs, Bernhard F, Wolff, H.H., Zillikens, Detlef, Grabbe, Jurgen (2005) Differential role for mitogen-activated protein kinases in IgE-dependent signalling in human peripheral blood basophils: in contrast to p38 MAPK, c-Jun N-terminal kinase is poorly expressed and does not appear to control mediator release. International Archives of Allergy and Immunology, 136 . pp. 329-339. ISSN 1018-2438. (doi:10.1159/000084226) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:4805)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL:
http://dx.doi.org/10.1159/000084226

Abstract

Background: Exposure of human basophils to allergens results in a rapid secretion of histamine, LTC4, IL-4 and IL-13, which dominate both the symptomology of allergic diseases and support the underlying Th2/IgE predominance associated with these reactions. The IgE-dependent release of these mediators in basophils crucially involves PI 3-kinase and the subsequent activation of p38 MAPK and ERK1B2. Here, we investigated the role of the third major member of the mitogen activated kinase family, namely the c-Jun amino terminal kinase (JNK), which is rapidly activated following IgE receptor cross-linking in murine mast cells. Methods: Human basophils were highly purified by magnetic cell sorting. The activities of various intracellular signaling components, in basophils that had been stimulated under various conditions, were assessed by Western blotting. Mediator secretions were also determined using either spectrofluorometric analysis ( histamine) or ELISA ( LTC4, IL-4 and IL-13). Results: Our results show that while JNK is moderately expressed in human basophils, it is not consistently phosphorylated upon anti-IgE stimulation. Phosphorylation of the transcription factor c-Jun, a downstream target of JNK, was also undetected in contrast to p38 MAPK and ERK1B2, which were clearly activated following anti-IgE stimulation of the cells. Additionally, inhibitors of the JNK pathway failed to prevent basophil mediator release and had no effect on the phosphorylation of p38 MAPK or ERK1B2 at concentrations which were specific for JNK blockade. Conclusions: These data suggest major differences in utilizing various members of the mitogen-activated kinase family in the signal transduction cascade of IgE-receptor-bearing cells. Copyright (C) 2005 S. Karger AG, Basel.

Item Type: Article
DOI/Identification number: 10.1159/000084226
Subjects: Q Science > QR Microbiology > QR180 Immunology
Divisions: Divisions > Division of Natural Sciences > Medway School of Pharmacy
Depositing User: Bernhard F. Gibbs
Date Deposited: 03 Sep 2008 21:50 UTC
Last Modified: 16 Nov 2021 09:43 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/4805 (The current URI for this page, for reference purposes)

University of Kent Author Information

Gibbs, Bernhard F.

Creator's ORCID:
CReDIT Contributor Roles:
  • Depositors only (login required):

Total unique views for this document in KAR since July 2020. For more details click on the image.