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The pancreas-specific protein disulphide-isomerase PDIp interacts with a hydroxyaryl group in ligands.

Klappa, Peter, Freedman, Robert B., Langenbuch, Martina, Lan, Michael S., Robinson, Gary K., Ruddock, Lloyd W. (2001) The pancreas-specific protein disulphide-isomerase PDIp interacts with a hydroxyaryl group in ligands. Biochemical Journal, 354 . pp. 553-559. ISSN 0264-6021. (doi:10.1042/0264-6021:3540553) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:44)

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Abstract

Using a cross-linking approach, we have recently demonstrated that radiolabelled model peptides or misfolded proteins specifically interact in vitro with two members of the protein disulphide- isomerase family, namely PDI and PDIp, in a crude extract from sheep pancreas microsomes. In addition, we have shown that tyrosine and tryptophan residues within a peptide are the recognition motifs for the binding to PDIp. Here we examine non-peptide ligands and present evidence that a hydroxyaryl group is a structural motif for the binding to PDIp; simple constructs containing this group and certain xenobiotics and phytoestrogens, which contain an unmodified hydroxyaryl group, can all efficiently inhibit peptide binding to PDIp. To our knowledge this is the first time that the recognition motif of a molecular chaperone or folding catalyst has been specified as a simple chemical structure.

Item Type: Article
DOI/Identification number: 10.1042/0264-6021:3540553
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Susan Davies
Date Deposited: 19 Dec 2007 17:49 UTC
Last Modified: 16 Nov 2021 09:38 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/44 (The current URI for this page, for reference purposes)

University of Kent Author Information

Klappa, Peter.

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Robinson, Gary K..

Creator's ORCID: https://orcid.org/0000-0003-2660-7778
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Ruddock, Lloyd W..

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