The pancreas-specific protein disulphide-isomerase PDIp interacts with a hydroxyaryl group in ligands.

Klappa, Peter and Freedman, Robert B. and Langenbuch, Martina and Lan, Michael S. and Robinson, Gary K. and Ruddock, Lloyd W. (2001) The pancreas-specific protein disulphide-isomerase PDIp interacts with a hydroxyaryl group in ligands. Biochemical Journal, 354 . pp. 553-559. ISSN 0264-6021. (Access to this publication is restricted)

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http://dx.doi.org/10.1042/0264-6021:3540553

Abstract

Using a cross-linking approach, we have recently demonstrated that radiolabelled model peptides or misfolded proteins specifically interact in vitro with two members of the protein disulphide- isomerase family, namely PDI and PDIp, in a crude extract from sheep pancreas microsomes. In addition, we have shown that tyrosine and tryptophan residues within a peptide are the recognition motifs for the binding to PDIp. Here we examine non-peptide ligands and present evidence that a hydroxyaryl group is a structural motif for the binding to PDIp; simple constructs containing this group and certain xenobiotics and phytoestrogens, which contain an unmodified hydroxyaryl group, can all efficiently inhibit peptide binding to PDIp. To our knowledge this is the first time that the recognition motif of a molecular chaperone or folding catalyst has been specified as a simple chemical structure.

Item Type: Article
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences > Protein Science Group
Depositing User: Sue Davies
Date Deposited: 19 Dec 2007 17:49
Last Modified: 24 Jun 2014 11:00
Resource URI: http://kar.kent.ac.uk/id/eprint/44 (The current URI for this page, for reference purposes)
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