Expression of erbB-4/HER-4 growth factor receptor isoforms in ovarian cancer

Gilmour, L.M. and Macleod, K.G. and McCaig, A. and Gullick, W.J. and Smyth, J.F. and Langdon, S.P. (2001) Expression of erbB-4/HER-4 growth factor receptor isoforms in ovarian cancer. Cancer Research, 61 (5). pp. 2169-2176. ISSN 0008-5472 . (Access to this publication is restricted)

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Abstract

Immunohistochemical expression of erbB4 protein was identified in 93% (49 of 53) of ovarian cancers using the HFR-1 antibody (targeted to the cytoplasmic domain of the erbB4 receptor) and in 89% (47 of 53) of ovarian cancers using the H4.77.16 antibody (targeted to the extracellular domain). Tumors of serous histology were more likely to express a higher level of erbB4 than endometrioid tumors, and for stage III serous tumors, long-term survival was associated with moderate to high coexpression of erbB4 and erbB2. Within ovarian cancer cell lines, high erbB4 expression was associated with cisplatin resistance. Using reverse transcription-PCR, the presence of multiple isoforms of erbB4 mRNA was identified in both ovarian primary tumors and cell lines. Splice variants in the juxtamembrane (JM-a and JM-d) and cytoplasmic (CT-a and CT-b) regions were identified in mRNA of both cell lines and primary tumors. The use of an anti-erbB4 blocking antibody suggested that erbB4 was not the mediator of the growth stimulatory effects of neuregulin in ovarian cancer cells and indeed could potentially antagonize this effect.

Item Type: Article
Uncontrolled keywords: Adenocarcinoma/genetics/metabolism/pathology Alternative Splicing Antibodies/pharmacology Base Sequence Blotting, Western Cell Division/drug effects/physiology Cystadenocarcinoma, Serous/genetics/metabolism/pathology Female Humans Immunohistochemistry Molecular Sequence Data Neoplasm Staging Neuregulin-1/pharmacology Ovarian Neoplasms/genetics/*metabolism/pathology Protein Isoforms Receptor, Epidermal Growth Factor/antagonists & inhibitors/*biosynthesis/genetics Reverse Transcriptase Polymerase Chain Reaction Signal Transduction/drug effects/physiology Tumor Cells, Cultured
Subjects: Q Science
R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences > Biomedical Research Group
Depositing User: Bill Gullick
Date Deposited: 12 Jun 2008 11:18
Last Modified: 05 Sep 2011 23:34
Resource URI: http://kar.kent.ac.uk/id/eprint/3994 (The current URI for this page, for reference purposes)
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