Expression of erbB-4/HER-4 growth factor receptor isoforms in ovarian cancer

Gilmour, Lynn M. R. and Macleod, Kenneth G. and McCaig, Alison and Gullick, William J. and Smyth, John F. and Langdon, Simon P. (2001) Expression of erbB-4/HER-4 growth factor receptor isoforms in ovarian cancer. Cancer Research, 61 (5). pp. 2169-2176. ISSN 0008-5472 . (Access to this publication is restricted)

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Abstract

Immunohistochemical expression of erbB4 protein was identified in 93% (49 of 53) of ovarian cancers using the HFR-1 antibody (targeted to the cytoplasmic domain of the erbB4 receptor) and in 89% (47 of 53) of ovarian cancers using the H4.77.16 antibody (targeted to the extracellular domain). Tumors of serous histology were more likely to express a higher level of erbB4 than endometrioid tumors, and for stage III serous tumors, long-term survival was associated with moderate to high coexpression of erbB4 and erbB2. Within ovarian cancer cell lines, high erbB4 expression was associated with cisplatin resistance. Using reverse transcription-PCR, the presence of multiple isoforms of erbB4 mRNA was identified in both ovarian primary tumors and cell lines. Splice variants in the juxtamembrane (JM-a and JM-d) and cytoplasmic (CT-a and CT-b) regions were identified in mRNA of both cell lines and primary tumors. The use of an anti-erbB4 blocking antibody suggested that erbB4 was not the mediator of the growth stimulatory effects of neuregulin in ovarian cancer cells and indeed could potentially antagonize this effect.

Item Type: Article
Uncontrolled keywords: Adenocarcinoma/genetics/metabolism/pathology Alternative Splicing Antibodies/pharmacology Base Sequence Blotting, Western Cell Division/drug effects/physiology Cystadenocarcinoma, Serous/genetics/metabolism/pathology Female Humans Immunohistochemistry Molecular Sequence Data Neoplasm Staging Neuregulin-1/pharmacology Ovarian Neoplasms/genetics/*metabolism/pathology Protein Isoforms Receptor, Epidermal Growth Factor/antagonists & inhibitors/*biosynthesis/genetics Reverse Transcriptase Polymerase Chain Reaction Signal Transduction/drug effects/physiology Tumor Cells, Cultured
Subjects: Q Science
R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences > Biomedical Research Group
Depositing User: Bill Gullick
Date Deposited: 12 Jun 2008 11:18
Last Modified: 07 Jul 2014 12:59
Resource URI: http://kar.kent.ac.uk/id/eprint/3994 (The current URI for this page, for reference purposes)
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