Biophysical studies of the translation initiation pathway using immobilised mRNA analogues

McCarthy, J.E.G. and Marsden, S. and von der Haar, T. (2007) Biophysical studies of the translation initiation pathway using immobilised mRNA analogues. In: Lorsch, J., ed. Translation Initiation: Reconstituted Systems and Biophysical Methods. Methods in Enzymology (Volume). Elsevier, pp. 247-264. ISBN 9780123739698. (Full text available)

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Abstract

A growing number of biophysical techniques use immobilized reactants for the quantitative study of macromolecular reactions. Examples of such approaches include surface plasmon resonance, atomic force microscopy, total reflection fluorescence microscopy, and others. Some of these methods have already been adapted for work with immobilized RNAs, thus making them available for the study of many reactions relevant to translation. Published examples include the study of kinetic parameters of protein/RNA interactions and the effect of helicases on RNA secondary structure. The common denominator of all of these techniques is the necessity to immobilize RNA molecules in a functional state on solid supports. In this chapter, we describe a number of approaches by which such immobilization can be achieved, followed by two specific examples for applications that use immobilized RNAs.

Item Type: Book section
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences > Protein Science Group
Depositing User: Tobias von der Haar
Date Deposited: 18 Jun 2008 12:57
Last Modified: 05 Sep 2011 23:34
Resource URI: http://kar.kent.ac.uk/id/eprint/3955 (The current URI for this page, for reference purposes)
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