Differing ADP Release Rates from Myosin Heavy Chain Isoforms Define the Shortening Velocity of Skeletal Muscle Fibers

Weiss, Stefan and Rossi, Rosetta and Pellegrino, Maria Antonietta and Bottinelli, Roberto and Geeves, Michael A. (2001) Differing ADP Release Rates from Myosin Heavy Chain Isoforms Define the Shortening Velocity of Skeletal Muscle Fibers. Journal of Biological Chemistry, 276 (49). pp. 45902-45908. ISSN 0021-9258. (The full text of this publication is not available from this repository)

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Abstract

To understand mammalian skeletal myosin isoform diversity, pure myosin isoforms of the four major skeletal muscle myosin types (myosin heavy chains I, IIA, IIX, and IIB) were extracted from single rat muscle fibers. The extracted myosin (1-2 microg/15-mm length) was sufficient to define the actomyosin dissociation reaction in flash photolysis using caged-ATP (Weiss, S., Chizhov, I., and Geeves, M. A. (2000) J. Muscle Res. Cell Motil. 21, 423-432). The ADP inhibition of the dissociation reaction was also studied to give the ADP affinity for actomyosin (K(AD)). The apparent second order rate constant of actomyosin dissociation gets faster (K(1)k(+2) = 0.17 -0.26 microm(-1) x s(-1)), whereas the affinity for ADP is weakened (250-930 microm) in the isoform order I, IIA, IIX, IIB. Both sets of values correlate well with the measured maximum shortening velocity (V(0)) of the parent fibers. If the value of K(AD) is controlled largely by the rate constant of ADP release (k(-AD)), then the estimated value of k(-AD) is sufficiently low to limit V(0). In contrast, [ATP]K(1)k(+2) at a physiological concentration of 5 mm ATP would be 2.5-6 times faster than k(-AD).

Item Type: Article
Additional information: 0021-9258 (Print) Journal Article Research Support, Non-U.S. Gov't
Uncontrolled keywords: Adenosine Diphosphate/*metabolism Adenosine Triphosphate/metabolism Animals Kinetics Muscle Fibers/metabolism/*physiology Muscle, Skeletal/metabolism/*physiology Myosin Heavy Chains/*metabolism Protein Isoforms/*metabolism Rats
Subjects: Q Science > QP Physiology (Living systems)
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: Michael Geeves
Date Deposited: 14 Sep 2008 08:14
Last Modified: 21 May 2014 07:35
Resource URI: http://kar.kent.ac.uk/id/eprint/3863 (The current URI for this page, for reference purposes)
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