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Mapping of the luteinizing hormone/choriogonadotropin receptor gene (LHCGR) to chicken chromosome 3

Ge, S F, Romanov, Michael N, Sharp, P J, Burt, David W, Paton, Ian R, Dunn, Ian C. (2001) Mapping of the luteinizing hormone/choriogonadotropin receptor gene (LHCGR) to chicken chromosome 3. Animal Genetics, 32 (1). p. 50. ISSN 0268-9146. (doi:10.1111/j.1365-2052.2001.0647i.pp.x) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:37571)

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Abstract

Source/description: The chicken luteinizing hormone/choriogonadotropin receptor gene has been cloned and sequenced. To produce markers for linkage mapping genomic DNA sequence from exon 7 to exon 9 including the intervening introns and exon was cloned and sequenced (EMBL accession number AJ289775). The intron-exon structure of this region, including exon 8 was the same as in mammalian LHCGR genes. A pair of primers for PCR which gave a single amplification product of 287 bp were then designed for the purposes of mapping. The sequence of the amplified product was confirmed by sequencing (97.6% sequence identity with AJ289775). Polymorphism was revealed by single strand conformation polymorphism (SSCP) analysis.

Chromosomal location: Segregation analysis indicated that the chicken luteinizing hormone/choriogonadotropin receptor gene was located on chromosome 3, placing it at position 45.35. The marker for the luteinizing hormone receptor gene (ROS0250) is tightly linked (phi = 0.0) to MCW0169 and ADL0177 with lod scores of 14.4 and 15.7, respectively. There was no evidence for conservation of gene order in this region of chicken chromosome 3 with human or mouse chromosomes.

Item Type: Article
DOI/Identification number: 10.1111/j.1365-2052.2001.0647i.pp.x
Projects: Candidate genes for reproductive efficiency in poultry (MAFF)
Uncontrolled keywords: chickens, receptors, chromosome mapping, genes, chromosomes, nucleotide sequences, exons, introns, luteinizing hormone; agriculture; genetics & heredity
Subjects: Q Science > QH Natural history > QH426 Genetics
S Agriculture > SF Animal culture
Divisions: Divisions > Division of Natural Sciences > Biosciences
Signature Themes: Food Systems, Natural Resources and Environment
Funders: China Scholarship Council (https://ror.org/04atp4p48)
Royal Society (https://ror.org/03wnrjx87)
Depositing User: Mike Romanov
Date Deposited: 12 Dec 2013 17:05 UTC
Last Modified: 08 Sep 2023 16:56 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/37571 (The current URI for this page, for reference purposes)

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