Thatcher, N.M. and Prior, M.J.W. and Morris, P.G. and Bachelard, H.S. (1999) Magnetic resonance spectroscopy studies on changes in cerebral calcium and zinc and the energy state caused by excitotoxic amino acids. Journal of Neurochemistry, 72 (6). pp. 2471-2478. ISSN 0022-3042.
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| Official URL http://dx.doi.org/10.1046/j.1471-4159.1999.0722471... |
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Abstract
Under control conditions, superfused hippocampal slices exhibited a significantly higher phosphocreatine (PCr)/ATP ratio than cortical slices; the evidence suggests that this is due to lower concentrations of ATP, rather than higher concentrations of PCr. Glutamate caused relatively rapid decreases in PCr and ATP levels to ~45%, accompanied or immediately followed by an increased free intracellular calcium concentration ([Ca 2+](i)) and the release of Zn 2+ in the cortex. In the hippocampus PCr and ATP decreased further to ~20% of control values, but the changes in [Ca 2+](i) and Zn 2+ content were slower. This is in contrast to the effects of depolarisation, which produced the same rapid changes in the energy state and [Ca 2+](i), with no detectable Zn 2+, in both tissues. NMDA causes effects similar to those of glutamate in the cortex (decreases in the energy state, increased [Ca 2+](i), and release of Zn 2+). Pretreatment of the cortex for I h with the NMDA blocker MK-801 prevented all of the observed effects of NMDA. In contrast, pretreatment with MK-801 had no detectable effect on the increase in [Ca 2+](i) or the decreases in PCr and ATP caused by glutamate, although it prevented the release of zinc. The results are discussed in relation to the function of the NMDA subtype of glutamate receptor in excitotoxicity.
| Item Type: | Article |
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| Uncontrolled keywords: | Calcium, Cerebral cortex, Energy state, Excitotoxicity, Hippocampus, Magnetic resonance spectroscopy, MK-801, Zinc, adenosine triphosphate, calcium ion, creatine phosphate, dizocilpine, excitatory amino acid, n methyl dextro aspartic acid receptor blocking agent, zinc, animal tissue, article, brain ischemia, brain slice, cerebrovascular disease, guinea pig, hippocampus, neurotransmission, nonhuman, phosphorus nuclear magnetic resonance, priority journal, Animals, Calcium, Cerebral Cortex, Dizocilpine Maleate, Energy Metabolism, Glutamic Acid, Guinea Pigs, Hippocampus, Magnetic Resonance Spectroscopy, Membrane Potentials, Mice, Neurotoxins, Phosphocreatine, Zinc |
| Subjects: | Q Science > QP Physiology (Living systems) > QP517 Biochemistry |
| Divisions: | Faculties > Science Technology and Medical Studies > School of Biosciences |
| Depositing User: | Nicola Cooper |
| Date Deposited: | 04 Dec 2012 16:35 |
| Last Modified: | 30 Apr 2013 14:08 |
| Resource URI: | http://kar.kent.ac.uk/id/eprint/32289 (The current URI for this page, for reference purposes) |
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