Encapsulation of superparamagnetic nanoparticles into red blood cells as new carriers of MRI contrast agents

Antonelli, Antonella and Sfara, Carla and Manuali, Elisabetta and Bruce, Ian J. and Magnani, Mauro (2011) Encapsulation of superparamagnetic nanoparticles into red blood cells as new carriers of MRI contrast agents. Nanomedicine, 6 (2). pp. 211-223. ISSN 1743-5889. (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.2217/Nnm.10.163

Abstract

Aims: The half-life of superparamagnetic iron oxide nanoparticles in the bloodstream is very short since they are rapidly taken up by the reticuloendothelial system. In this article, we report the encapsulation of different magnetic nanoparticles into human erythrocytes to increase their blood circulation time. Materials & methods: Newly synthesized and commercially available nanoparticles were evaluated for the encapsulation into red blood cells through the transient opening of membrane pores by controlled hypotonic dialysis and successive isotonic resealing and reannealing of cells. Results: Commercial superparamagnetic iron oxide nanoparticles (SHU 555A, AMI 227 and PMP-50) dextran or carboxydextran coated can be successfully loaded into red blood cells; similarly, some of the new nanomaterials, such as Np-1 nanoparticles dispersed in the Disperbyk®-190 agent, can be efficiently encapsulated into red blood cells. Conclusion: A careful consideration of magnetic nanoparticles parameters, such as size, synthesis protocols, coating and/or dispersant agents, is required in order to obtain efficient loading through the cell membrane pores.

Item Type: Article
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: Sue Davies
Date Deposited: 07 Nov 2012 09:55
Last Modified: 08 Apr 2014 08:12
Resource URI: http://kar.kent.ac.uk/id/eprint/32107 (The current URI for this page, for reference purposes)
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