Rossman, Jeremy S. and Jing, Xianghong and Leser, George P. and Lamb, Robert A. (2010) Influenza Virus M2 Protein Mediates ESCRT-Independent Membrane Scission. Cell, 142 (6). pp. 902-913. ISSN 00928674.
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| Official URL http://dx.doi.org/10.1016/j.cell.2010.08.029 |
Abstract
Many viruses utilize host ESCRT proteins for budding; however, influenza virus budding is thought to be ESCRT-independent. In this study we have found a role for the influenza virus M2 proton-selective ion channel protein in mediating virus budding. We observed that a highly conserved amphipathic helix located within the M2 cytoplasmic tail mediates a cholesterol-dependent alteration in membrane curvature. The 17 amino acid amphipathic helix is sufficient for budding into giant unilamellar vesicles, and mutation of this sequence inhibited budding of transfected M2 protein in vivo. We show that M2 localizes to the neck of budding virions and that mutation of the M2 amphipathic helix results in failure of the virus to undergo membrane scission and virion release. These data suggest that M2 mediates the final steps of budding for influenza viruses, bypassing the need for host ESCRT proteins.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science |
| Divisions: | Faculties > Science Technology and Medical Studies > School of Biosciences |
| Depositing User: | Sue Davies |
| Date Deposited: | 08 Oct 2012 15:53 |
| Last Modified: | 30 Jan 2013 12:17 |
| Resource URI: | http://kar.kent.ac.uk/id/eprint/31396 (The current URI for this page, for reference purposes) |
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