Chen, L. and Hughes, R. A. and Baines, A. J. and Conboy, J. and Mohandas, N. and An, X. (2011) Protein 4.1R regulates cell adhesion, spreading, migration and motility of mouse keratinocytes by modulating surface expression of 1 integrin. Journal of Cell Science, 124 (14). pp. 2478-2487. ISSN 0021-9533.
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Protein 4.1R is a membrane-cytoskeleton adaptor protein that has diverse roles in controlling the cell surface expression and/or function of transmembrane proteins, and in organizing F-actin. 4.1R is expressed in keratinocytes, but its role in these cells has not been explored. Here, we have investigated the role of 4.1R in skin using 4.1R−/− mice. Cell adhesion, spreading, migration and motility were significantly impaired in 4.1R−/− keratinocytes, and 4.1R−/− mice exhibited defective epidermal wound healing. Cultured 4.1R−/− keratinocytes on fibronectin failed to form actin stress fibres and focal adhesions. Furthermore, in the absence of 4.1R, the surface expression, and consequently the activity of β1 integrin were reduced. These data enabled the identification of a functional role for protein 4.1R in keratinocytes by modulating the surface expression of β1 integrin, possibly through a direct association between 4.1R and β1 integrin.
|Uncontrolled keywords:||4.1R; β1 integrin; Cell motility; Keratinocyte|
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||Sue Davies|
|Date Deposited:||08 Oct 2012 10:18|
|Last Modified:||30 Jan 2013 09:39|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/31345 (The current URI for this page, for reference purposes)|
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