Neutralizing monoclonal antibodies to different clades of Influenza A H5N1 viruses

Oh, S. and Selleck, P. and Temperton, N.J. and Chan, P.K.S. and Capecchi, B. and Manavis, J. and Higgins, G. and Burrell, C.J. and Kok, T. (2009) Neutralizing monoclonal antibodies to different clades of Influenza A H5N1 viruses. Journal of Virological Methods, 157 (2). pp. 161-167. ISSN 01660934 (ISSN) . (The full text of this publication is not available from this repository)

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Abstract

Four IgG 1κ monoclonal antibodies (mAbs) against Influenza A/Chicken/Vietnam/8/2004 (H5N1) virus are described. Three of these showed neutralizing activities against H5N1 strains from clades 1, 2 and 3 using a retroviral pseudotype or live virus microneutralization assay. In the pseudotype assay, the IC 90 neutralizing titre range was >1600-51,200, and with the microneutralization was 80 ≥ 10,240. MAb 1C1 showed strong neutralizing activities in both assays. All four mAbs reacted specifically to the immunogen by immunohistochemical staining and to A/Hong Kong/483/1997 (H5N1) and A/Thailand/1(KAN-1)/2004 (H5N1)-infected MDCK cells by immunofluorescence. ELISA titrations of the mAbs showed specificity for H5N1 haemagglutinin (HA) and no cross-reactivity to 15 other Influenza A subtypes. Only mAbs 1C1 and the non-neutralizing 1F7 reacted with HA 1, the cleaved subunit of HA, by Western blot. These results suggest that the mAbs recognize distinct or overlapping epitopes and will be useful reagents for construction of specific rapid point-of-care assays or for therapeutic use.

Item Type: Article
Uncontrolled keywords: H5N1, Haemagglutinin, Influenza, Monoclonal antibodies, Neutralization, Pseudotype, antigen, epitope, hemagglutinin, monoclonal antibody, neutralizing antibody, reagent, animal cell, antibody production, antibody titer, article, cell strain, cladistics, controlled study, cross reaction, enzyme linked immunosorbent assay, Hong Kong, human, immunofluorescence, immunogenicity, immunohistochemistry, Influenza virus A H5N1, nonhuman, priority journal, pseudotyping, Retrovirus, sensitivity and specificity, Thailand, virus neutralization, Western blotting, Animals, Antibodies, Monoclonal, Antibodies, Viral, Chickens, Cross Reactions, Hemagglutinins, Viral, Immunoglobulin G, Influenza A Virus, H5N1 Subtype, Inhibitory Concentration 50, Neutralization Tests
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Faculties > Science Technology and Medical Studies > Medway School of Pharmacy
Depositing User: Nigel Temperton
Date Deposited: 02 Aug 2012 10:03
Last Modified: 03 Aug 2012 11:52
Resource URI: http://kar.kent.ac.uk/id/eprint/29993 (The current URI for this page, for reference purposes)
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