Adjuvant-free immunization with hemagglutinin-Fc fusion proteins as an approach to influenza vaccines

Loureiro, Silvia and Ren, Junyuan and Phapugrangkul, Pongsathon and Colaco, Camilo A. and Bailey, Christopher R. and Shelton, Holly and Molesti, Eleonora and Temperton, Nigel J. and Barclay, Wendy S and Jones, Ian M. (2011) Adjuvant-free immunization with hemagglutinin-Fc fusion proteins as an approach to influenza vaccines. Journal of Virology, 85 (6). pp. 3010-3014. ISSN 0022-538X. (Full text available)

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Abstract

The hemagglutinins (HAs) of human H1 and H3 influenza viruses and avian H5 influenza virus were produced as recombinant fusion proteins with the human immunoglobulin Fc domain. Recombinant HA-human immunoglobulin Fc domain (HA-HuFc) proteins were secreted from baculovirus-infected insect cells as glycosylated oligomer HAs of the anticipated molecular mass, agglutinated red blood cells, were purified on protein A, and were used to immunize mice in the absence of adjuvant. Immunogenicity was demonstrated for all subtypes, with the serum samples demonstrating subtype-specific hemagglutination inhibition, epitope specificity similar to that seen with virus infection, and neutralization. HuFc-tagged HAs are potential candidates for gene-to-vaccine approaches to influenza vaccination. Copyright © 2011, American Society for Microbiology. All Rights Reserved.

Item Type: Article
Uncontrolled keywords: epitope, hemagglutin Fc receptor fusion protein, hybrid protein, influenza vaccine, protein A, unclassified drug, article, cross reaction, drug synthesis, hemagglutination inhibition, immunogenicity, influenza, influenza vaccination, molecular weight, nonhuman, priority journal, protein purification, seroconversion, virus hemagglutination, virus infection, virus inhibition, virus neutralization, Animals, Antibodies, Viral, Baculoviridae, Cell Line, Genetic Vectors, Hemagglutination Inhibition Tests, Hemagglutinin Glycoproteins, Influenza Virus, Humans, Immunoglobulin Fc Fragments, Influenza A virus, Influenza Vaccines, Insects, Mice, Neutralization Tests, Recombinant Fusion Proteins, Vaccines, Synthetic, Aves, Hexapoda, Mus, Orthomyxoviridae
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Faculties > Science Technology and Medical Studies > Medway School of Pharmacy
Depositing User: Nigel Temperton
Date Deposited: 02 Aug 2012 10:43
Last Modified: 17 Jun 2014 08:25
Resource URI: http://kar.kent.ac.uk/id/eprint/29988 (The current URI for this page, for reference purposes)
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