Antigenic drift in H5N1 avian influenza virus in poultry is driven by mutations in major antigenic sites of the hemagglutinin molecule analogous to those for human influenza virus

Cattoli, Giovanni and Milani, Adelaide and Temperton, Nigel J. and Zecchin, Bianca and Buratin, Alessandra and Molesti, Eleonora and Aly, Mona Meherez and Arafa, Abdel and Capua, Ilaria (2011) Antigenic drift in H5N1 avian influenza virus in poultry is driven by mutations in major antigenic sites of the hemagglutinin molecule analogous to those for human influenza virus. Journal of Virology, 85 (17). pp. 8718-8724. ISSN 0022-538X. (Full text available)

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http://dx.doi.org/10.1128/JVI.02403-10

Abstract

H5N1 highly pathogenic avian influenza virus has been endemic in poultry in Egypt since 2008, notwithstanding the implementation of mass vaccination and culling of infected birds. Extensive circulation of the virus has resulted in a progressive genetic evolution and an antigenic drift. In poultry, the occurrence of antigenic drift in avian influenza viruses is less well documented and the mechanisms remain to be clarified. To test the hypothesis that H5N1 antigenic drift is driven by mechanisms similar to type A influenza viruses in humans, we generated reassortant viruses, by reverse genetics, that harbored molecular changes identified in genetically divergent viruses circulating in the vaccinated population. Parental and reassortant phenotype viruses were antigenically analyzed by hemagglutination inhibition (HI) test and microneutralization (MN) assay. The results of the study indicate that the antigenic drift of H5N1 in poultry is driven by multiple mutations primarily occurring in major antigenic sites at the receptor binding subdomain, similarly to what has been described for human influenza H1 and H3 subtype viruses. © 2011, American Society for Microbiology.

Item Type: Article
Uncontrolled keywords: Influenza virus hemagglutinin, amino acid sequence, amino acid substitution, antigenic drift, antigenicity, article, controlled study, genetic reassortment, hemagglutination inhibition test, Influenza virus A H5N1, nonhuman, nucleotide sequence, phenotype, poultry, priority journal, sequence alignment, virus characterization, virus mutation, virus neutralization, Amino Acid Sequence, Animals, Antigens, Viral, Egypt, Hemagglutination Inhibition Tests, Hemagglutinin Glycoproteins, Influenza Virus, Humans, Influenza A Virus, H5N1 Subtype, Influenza in Birds, Models, Molecular, Molecular Sequence Data, Mutation, Missense, Neutralization Tests, Poultry, Reassortant Viruses, Sequence Alignment, Aves, Avian influenza virus, human influenza virus, Orthomyxoviridae
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Faculties > Science Technology and Medical Studies > Medway School of Pharmacy
Depositing User: Nigel Temperton
Date Deposited: 02 Aug 2012 09:57
Last Modified: 10 Jun 2014 08:09
Resource URI: http://kar.kent.ac.uk/id/eprint/29986 (The current URI for this page, for reference purposes)
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