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The transcription factor Flo8 mediates CO2 sensing in the human fungal pathogen Candida albicans

Du, Han, Guan, Guobo, Xie, Jing, Cottier, Fabien, Sun, Yuan, Jia, Wei, Mühlschlegel, Fritz A., Huang, Guanghua (2012) The transcription factor Flo8 mediates CO2 sensing in the human fungal pathogen Candida albicans. Molecular Biology of the Cell, 23 (14). pp. 2692-2701. ISSN 1059-1524. (doi:10.1091/mbc.E12-02-0094) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:29890)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL:
http://dx.doi.org/10.1091/mbc.E12-02-0094

Abstract

Physiological levels of CO2 have a profound impact on prominent biological attributes of the major fungal pathogen of humans, Candida albicans. Elevated CO2 induces filamentous growth and promotes white-to-opaque switching. However, the underlying molecular mechanisms of CO2 sensing in C. albicans are insufficiently understood. Here we identify the transcription factor Flo8 as a key regulator of CO2-induced morphogenesis in C. albicans by screening a gene null mutant library. We show that Flo8 is required for CO2-induced white-to-opaque switching, as well as for filamentous growth. Ectopic expression of FLO8 hypersensitizes C. albicans cells to the elevated CO2 levels. Furthermore, we demonstrate that CO2 signaling in C. albicans involves two pathways: the already reported cAMP/protein kinase A and another major one that is unidentified. The two pathways converge on the transcription factor Flo8, which is the master regulator of CO2 sensing in C. albicans and plays a critical role in regulation of white-to-opaque switching and filamentous growth. Our findings provide new insights into the understanding of CO2 sensing in pathogenic fungi that have important implications for higher organisms.

Item Type: Article
DOI/Identification number: 10.1091/mbc.E12-02-0094
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Susan Davies
Date Deposited: 17 Jul 2012 09:39 UTC
Last Modified: 16 Nov 2021 10:07 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/29890 (The current URI for this page, for reference purposes)

University of Kent Author Information

Mühlschlegel, Fritz A..

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