Alberini, I. and Del Tordello, E. and Fasolo, A. and Temperton, N.J. and Galli, G. and Gentile, C. and Montomoli, E. and Hilbert, A.K. and Banzhoff, A. and Del Giudice, G. and Donnelly, J.J. and Rappuoli, R. and Capecchi, B. (2009) Pseudoparticle neutralization is a reliable assay to measure immunity and cross-reactivity to H5N1 influenza viruses. Vaccine, 27 (43). pp. 5998-6003. ISSN 0264-410X.
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The standard serological methods present limitations for the measurement of immunity against H5N1 influenza strains. The hemagglutination inhibition (HI) assay lacks sensitivity and requires standardization, while the viral micro-neutralization (MN) assay needs handling of live virus. We produced pseudoparticles expressing hemagglutinin from clades 1 or 2 H5N1 in order to measure neutralizing antibodies in human sera after prime-boost vaccination with plain or MF59-adjuvanted H5N1 clade 1 subunit vaccines. Titers measured by pseudoparticle neutralization (PPN) assay significantly correlated with those measured by HI, single radial haemolysis or MN, with a PPN titer of 1:357 corresponding to an MN titer of 1:80. Notably, results from the PPN assay, confirm that MF59-H5N1 vaccine induces potent and long-lasting neutralizing antibody responses not only against the vaccine strain, but also against several heterologous clade 2 strains. Overall, the PPN assay represents a valid alternative to conventional serological methods for the evaluation of H5N1 vaccine immunogenicity. Â© 2009 Elsevier Ltd. All rights reserved.
|Uncontrolled keywords:||Avian influenza virus, Cross-clade neutralization, H5N1, Pandemic vaccine, Pseudoparticle neutralization assay, Serological methods, avian influenza vaccine, n acetylmuramylalanyl dextro isoglutaminylalanyl dipalmitoylphosphatidylethanolamine, neutralizing antibody, virus hemagglutinin, article, avian influenza, blood, blood sampling, cross reaction, hemagglutination inhibition test, hemolysis, human, immunity, Influenza virus A H5N1, priority journal, serodiagnosis, virus strain, virus titration, Adult, Antibodies, Viral, Cross Reactions, Hemagglutination Inhibition Tests, Hemagglutinin Glycoproteins, Influenza Virus, Humans, Influenza A Virus, H5N1 Subtype, Influenza Vaccines, Influenza, Human, Neutralization Tests|
|Subjects:||Q Science > QR Microbiology > QR355 Virology|
|Divisions:||Faculties > Science Technology and Medical Studies > Medway School of Pharmacy|
|Depositing User:||Nigel Temperton|
|Date Deposited:||02 Aug 2012 06:51|
|Last Modified:||03 Aug 2012 09:59|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/29400 (The current URI for this page, for reference purposes)|
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