A neutralizing antibody selected from plasma cells that binds to group 1 and group 2 influenza A hemagglutinins

Corti, Davide and Voss, Jarrod and Gamblin, Steven J. and Codoni, Giosiana and Macagno, Annalisa and Jarrossay, David and Vachieri, Sebastien G. and Pinna, Debora and Minola, Andrea and Vanzetta, Fabrizia and Silacci, Chiara and Fernandez-Rodriguez, Blanca M. and Agatic, Gloria and Bianchi, Siro and Giacchetto-Sasselli, Isabella and Calder, Lesley and Sallusto, Federica and Collins, Patrick and Haire, Lesley F. and Temperton, Nigel J. and Langedijk, Johannes P. M. and Skehel, John J. and Lanzavecchia, Antonio (2011) A neutralizing antibody selected from plasma cells that binds to group 1 and group 2 influenza A hemagglutinins. Science, 333 (6044). pp. 850-856. ISSN 0036-8075. (The full text of this publication is not available from this repository)

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Abstract

The isolation of broadly neutralizing antibodies against influenza A viruses has been a long-sought goal for therapeutic approaches and vaccine design. Using a single-cell culture method for screening large numbers of human plasma cells, we isolated a neutralizing monoclonal antibody that recognized the hemagglutinin (HA) glycoprotein of all 16 subtypes and neutralized both group 1 and group 2 influenza A viruses. Passive transfer of this antibody conferred protection to mice and ferrets. Complexes with HAs from the group 1 H1 and the group 2 H3 subtypes analyzed by x-ray crystallography showed that the antibody bound to a conserved epitope in the F subdomain. This antibody may be used for passive protection and to inform vaccine design because of its broad specificity and neutralization potency.

Item Type: Article
Uncontrolled keywords: Influenza virus hemagglutinin, monoclonal antibody F16, monoclonal antibody F16v3, neutralizing antibody, unclassified drug, antibody, biochemistry, crystallography, cytology, disease treatment, influenza, neutralization, plasma, protein, virus, X-ray spectroscopy, animal experiment, animal model, antibody isolation, article, cell culture, controlled study, drug design, ferret, influenza A, Influenza virus A, mouse, nonhuman, passive immunization, plasma cell, priority journal, X ray crystallography, Animals, Antibodies, Neutralizing, Antibodies, Viral, Antibody Specificity, Antigens, Viral, Cells, Cultured, Cross Reactions, Crystallography, X-Ray, Epitopes, Ferrets, Glycosylation, Hemagglutinin Glycoproteins, Influenza Virus, Humans, Hydrophobic and Hydrophilic Interactions, Immunization, Passive, Immunoglobulin Variable Region, Influenza A virus, Influenza A Virus, H1N1 Subtype, Influenza B virus, Influenza, Human, Mice, Models, Molecular, Molecular Sequence Data, Orthomyxoviridae Infections, Plasma Cells, Protein Multimerization, Protein Structure, Secondary, Influenza A virus, Mus, Mustela
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Faculties > Science Technology and Medical Studies > Medway School of Pharmacy
Depositing User: Nigel Temperton
Date Deposited: 02 Aug 2012 06:56
Last Modified: 09 Jun 2014 15:56
Resource URI: http://kar.kent.ac.uk/id/eprint/29397 (The current URI for this page, for reference purposes)
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