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The isolation of broadly neutralizing antibodies against influenza A viruses has been a long-sought goal for therapeutic approaches and vaccine design. Using a single-cell culture method for screening large numbers of human plasma cells, we isolated a neutralizing monoclonal antibody that recognized the hemagglutinin (HA) glycoprotein of all 16 subtypes and neutralized both group 1 and group 2 influenza A viruses. Passive transfer of this antibody conferred protection to mice and ferrets. Complexes with HAs from the group 1 H1 and the group 2 H3 subtypes analyzed by x-ray crystallography showed that the antibody bound to a conserved epitope in the F subdomain. This antibody may be used for passive protection and to inform vaccine design because of its broad specificity and neutralization potency.
|Uncontrolled keywords:||Influenza virus hemagglutinin, monoclonal antibody F16, monoclonal antibody F16v3, neutralizing antibody, unclassified drug, antibody, biochemistry, crystallography, cytology, disease treatment, influenza, neutralization, plasma, protein, virus, X-ray spectroscopy, animal experiment, animal model, antibody isolation, article, cell culture, controlled study, drug design, ferret, influenza A, Influenza virus A, mouse, nonhuman, passive immunization, plasma cell, priority journal, X ray crystallography, Animals, Antibodies, Neutralizing, Antibodies, Viral, Antibody Specificity, Antigens, Viral, Cells, Cultured, Cross Reactions, Crystallography, X-Ray, Epitopes, Ferrets, Glycosylation, Hemagglutinin Glycoproteins, Influenza Virus, Humans, Hydrophobic and Hydrophilic Interactions, Immunization, Passive, Immunoglobulin Variable Region, Influenza A virus, Influenza A Virus, H1N1 Subtype, Influenza B virus, Influenza, Human, Mice, Models, Molecular, Molecular Sequence Data, Orthomyxoviridae Infections, Plasma Cells, Protein Multimerization, Protein Structure, Secondary, Influenza A virus, Mus, Mustela|
|Subjects:||Q Science > QR Microbiology > QR355 Virology|
|Divisions:||Faculties > Science Technology and Medical Studies > Medway School of Pharmacy|
|Depositing User:||Nigel Temperton|
|Date Deposited:||02 Aug 2012 06:56|
|Last Modified:||03 Aug 2012 11:29|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/29397 (The current URI for this page, for reference purposes)|
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