Grotzkyj-Giorgi, Margherita and Howland, K. and Martin, C. and Bonner, A.B. (2012) A Novel HPLC Method for the Concurrent Analysis and Quantitation of Seven Water-Soluble Vitamins in Biological Fluids (Plasma and Urine): A Validation Study and Application. The Scientific World Journal, 2012 . pp. 1-8. ISSN 1537-744X.
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Available under License Creative Commons Attribution.
An HPLC method was developed and validated for the concurrent detection and quantitation of seven water-soluble vitamins (C, B1, B2, B5, B6, B9, B12) in biological matrices (plasma and urine). Separation was achieved at 30°C on a reversed-phase C18-A column using combined isocratic and linear gradient elution with a mobile phase consisting of 0.01% TFA aqueous and 100% methanol. Total run time was 35 minutes. Detection was performed with diode array set at 280 nm. Each vitamin was quantitatively determined at its maximum wavelength. Spectral comparison was used for peak identification in real samples (24 plasma and urine samples from abstinent alcohol-dependent males). Interday and intraday precision were <4% and <7%, respectively, for all vitamins. Recovery percentages ranged from 93% to 100%.
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences
Faculties > Science Technology and Medical Studies > School of Biosciences > Protein Science Group
|Depositing User:||Sue Davies|
|Date Deposited:||27 Mar 2012 13:18|
|Last Modified:||12 Jun 2012 12:44|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/29204 (The current URI for this page, for reference purposes)|
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