A role for the actin cytoskeleton in cell death and aging in yeast.

Gourlay, Campbell W. and Carpp, Lindsay N. and Timpson, Paul and Winder, Steven J. and Ayscough, Kathryn R. (2004) A role for the actin cytoskeleton in cell death and aging in yeast. Journal of Cell Biology, 164 (6). pp. 803-809. ISSN 0021-9525 . (Access to this publication is restricted)

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Abstract

Several determinants of aging, including metabolic capacity and genetic stability, are recognized in both yeast and humans. However, many aspects of the pathways leading to cell death remain to be elucidated. Here we report a role for the actin cytoskeleton both in cell death and in promoting longevity. We have analyzed yeast strains expressing mutants with either increased or decreased actin dynamics. We show that decreased actin dynamics causes depolarization of the mitochondrial membrane and an increase in reactive oxygen species (ROS) production, resulting in cell death. Important, however, is the demonstration that increasing actin dynamics, either by a specific actin allele or by deletion of a gene encoding the actin-bundling protein Scp1p, can increase lifespan by over 65%. Increased longevity appears to be due to these cells producing lower than wild-type levels of ROS. Homology between Scp1p and mammalian SM22/transgelin, which itself has been isolated in senescence screens, suggests a conserved mechanism linking aging to actin stability.

Item Type: Article
Uncontrolled keywords: actin; senescence; Scp1; apoptosis; ROS
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences > Cell & Developmental Biology Group
Depositing User: Sue Davies
Date Deposited: 19 Dec 2007 17:46
Last Modified: 16 Jun 2014 10:52
Resource URI: http://kar.kent.ac.uk/id/eprint/29 (The current URI for this page, for reference purposes)
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