PCR-SSCP analysis of Fusarium diversity in asparagus decline in Japan

Nahiyan, Abu Shamim Mohammad and Boyer, Louisa Robinson and Jeffries, P. and Matsubara, Yoh-ichi (2011) PCR-SSCP analysis of Fusarium diversity in asparagus decline in Japan. European Journal of Plant Pathology, 130 (2). pp. 197-203. ISSN 0929-1873. (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1007/s10658-011-9745-y

Abstract

The diversity of Fusarium populations in asparagus (Asparagus officinalis L.) decline fields in Japan was estimated by PCR-SSCP (single-stranded conformational polymorphism) analysis of the ITS2 regions of the nuclear rRNA genes. This method was used to rapidly and objectively identify pathogens associated with roots of plants showing symptoms of asparagus decline collected from fields in five regions across Japan. Over 651 fusarial isolates were obtained, and were easily differentiated into three principal species. Fusarium oxysporum f. sp. asparagi was most frequently isolated from the domestic five regions (68%), whereas Fusarium proliferatum (28.6%) was less frequent. Fusarium solani was found much rarely (2.5%). The frequency of isolation of Fusarium proliferatum increased gradually from the north to the south of Japan, though considerable differences were found between fields in each region, as well as regional differences among the Fusarium populations. Most of the fusarial isolates were highly pathogenic in vitro. These results reveal that Fusarium oxysporum f. sp. asparagi and Fusarium proliferatum are important biotic factors which lead to asparagus decline in Japan.

Item Type: Article
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: Sue Davies
Date Deposited: 23 Nov 2011 11:39
Last Modified: 25 Nov 2011 09:24
Resource URI: http://kar.kent.ac.uk/id/eprint/28473 (The current URI for this page, for reference purposes)
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