A quantitative approach to nitric oxide inhibition of terminal oxidases of the respiratory chain.

Mason, Maria G. and Holladay, Rebecca S. and Nicholls, Peter J. and Shepherd, Mark and Cooper, Chris E (2008) A quantitative approach to nitric oxide inhibition of terminal oxidases of the respiratory chain. Methods in Enzymology, 437 . pp. 135-159. ISSN 0076-6879. (The full text of this publication is not available from this repository)

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Official URL
http://dx.doi.org/10.1016/S0076-6879(07)37008-0

Abstract

Inhibition of terminal respiratory oxidases by nitric oxide (NO) plays important physiological roles in signaling and host defense. Using a bacterial quinol oxidase and mitochondrial cytochrome c oxidase, this chapter describes simple polarographic methods to quantify the kinetic characteristics of inhibition by NO. This chapter points out the inherent pitfalls of both experimental design and data analysis and compares alternative methods. Additionally, it describes a system designed to acquire polarographic and spectral data simultaneously to permit identification of spectral intermediates under defined conditions.

Item Type: Article
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: Mark Shepherd
Date Deposited: 01 Sep 2011 15:57
Last Modified: 30 May 2014 13:19
Resource URI: http://kar.kent.ac.uk/id/eprint/28099 (The current URI for this page, for reference purposes)
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