Mason, M.G. and Holladay, R.S. and Nicholls, P. and Shepherd, Mark and Cooper, C.E. (2008) A quantitative approach to nitric oxide inhibition of terminal oxidases of the respiratory chain. Methods in Enzymology, 437 . pp. 135-159. ISSN 0076-6879.
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Inhibition of terminal respiratory oxidases by nitric oxide (NO) plays important physiological roles in signaling and host defense. Using a bacterial quinol oxidase and mitochondrial cytochrome c oxidase, this chapter describes simple polarographic methods to quantify the kinetic characteristics of inhibition by NO. This chapter points out the inherent pitfalls of both experimental design and data analysis and compares alternative methods. Additionally, it describes a system designed to acquire polarographic and spectral data simultaneously to permit identification of spectral intermediates under defined conditions.
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||Mark Shepherd|
|Date Deposited:||01 Sep 2011 15:57|
|Last Modified:||25 Jun 2012 10:59|
|Resource URI:||http://kar.kent.ac.uk/id/eprint/28099 (The current URI for this page, for reference purposes)|
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